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Cloning and DNA-binding properties of ethylene response factor, LeERF1 and LeERF2, in tomato

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Abstract

Two new genes, LeERF1 andLeERF2, were isolated from a tomato (Lycopersicon esculentum cv. Lichun) cDNA library. Phylogenetic analysis indicated that they encoded Ethylene Responsive Element Binding Proteins (EREBPs), characterized by a conserved ERF (ethylene response factor) domain of specific binding plant cis-acting elements GCC box. Both LeERF1 and LeERF2 proteins were obtained via prokaryotic expression and purification. Electrophoretic mobility shift assay showed that LeERF1 and LeERF2 protein could bind to the promoter of the NP24 gene coding for pathogenesis-related protein osmotin precursor but not the mutant promoter where its GCC box was deleted. Polyclonal antibodies of LeERF1 and LeERF2 blocked their binding in vitro.

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Authors and Affiliations

  1. Department of Biotechnology, the College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China

    Zhang Hongxing, Zhu Benzhong, Yu Bianyun, Hao Yanling, Fu Daqi, Xu Wentao & Luo Yunbo

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  1. Zhang Hongxing
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  2. Zhu Benzhong
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  3. Yu Bianyun
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  4. Hao Yanling
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  5. Fu Daqi
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  6. Xu Wentao
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  7. Luo Yunbo
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Corresponding author

Correspondence to Luo Yunbo.

Additional information

Revisions requested 4 January 2005; Revisions received 28 January 2005

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Hongxing, Z., Benzhong, Z., Bianyun, Y. et al. Cloning and DNA-binding properties of ethylene response factor, LeERF1 and LeERF2, in tomato. Biotechnol Lett 27, 423–428 (2005). https://doi.org/10.1007/s10529-005-1777-y

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  • DOI: https://doi.org/10.1007/s10529-005-1777-y

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