Abstract
Improvement of the fermentation efficiency of poly-β-hydroxybutyrate (PHB) may make it competitive with chemically synthesized petroleum-based polymers. One step toward this is optimization of fluid dispersion and the feed rates to a fed-batch bioreactor. In a recent study using a fermentation model, dispersion corresponding to a Peclet number of ≈20 was shown to maximize the productivity of PHB. Here further improvement has been investigated using neural optimization. A comparison of seven neural topologies has shown that while feed-forward and radial basis neural networks are computationally efficient, recurrent networks generate higher concentrations of PHB. All networks enhanced the productivity by 16–93% over model-based optimization.
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References
HW Blanch DS Clark (1996) Biochemical Engineering Marcell Dekker New York
G Braunegg G Lefebre KF Genser (1998) ArticleTitlePolyhydroxyalkanoates, biopolymers from renewable resources: physiological and engineering aspects J. Biotechnol. 65 127–161
VCP Chen DK Rollins (2000) ArticleTitleIssues regarding artificial neural network modeling of reactors and fermenters Bioproc. Biosyst. Eng. 22 85–93
GF Froment KB Bischoff (1990) Chemical Reactor Analysis and Design John Wiley New York.
KG Gadkar FJ Doyle SuffixIII TJ Crowley JD Varner (2003) ArticleTitleCybernetic model predictive control of a continuous bioreactor with cell recycle Biotechnol. Prog. 19 1487–1497
MT Hagan Demuth MH Beale (1996) Neural Network Design PWS Publ. MA. Boston
T Katoh D Yuguchi H Yoshii H Shi K Shimizu (1999) ArticleTitleDynamics and modelling on fermentative production of poly(β-hydroxybutyric acid) from sugars via lactate by a mixed culture of Lactobacillus delbrueckii and Alcaligenes eutrophus J. Biotechnol. 67 113–134
BS Kim SC Lee SY Lee HN Chang YK Chang SI Woo (1994) ArticleTitleProduction of poly(3-hydroxybutyric acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control Biotechnol. Bioeng. 43 892–898
SY Lee HN Chang (1995) ArticleTitleProduction of poly(hydroxyalkanoic acid) Adv. Biochem. Eng. Biotechnol. 52 27–58
JH Lee HC Lim J Hong (1997) ArticleTitleApplication of nonsingular transformation to on-line optimal control of poly-β-hydroxybutyrate fermentation J. Biotechnol. 55 135–150
PR Patnaik (1996) ArticleTitlePreliminary screening of neural network configurations for bioreactor applications Biotechnol. Tech. 10 967–970
PR Patnaik (1998) Neural network applications to fermentation processes G. Subramanian (Eds) Bioseparation and Bioprocessing Weinheim Wiley-VCH
PR Patnaik (1999) ArticleTitleNeural control of an imperfectly mixed fed-batch bioreactor for recombinant β-galactosidase Biochem. Eng. J. 3 113–120
PR Patnaik (2001) ArticleTitleA simulation study of dynamic neural filtering and control of a fed-batch bioreactor under nonideal conditions Chem. Eng. J. 84 533–541
D Ramkrishna DS Kompala GT Tsao (1987) ArticleTitleAre microbes optimal strategists? Biotechnol Prog. 3 121–126
CAM Riascos Pinto (2004) ArticleTitleOptimal control of bioreactors: a simultaneous approach for complex systems Chem. Eng. J. 99 23–34
JA Routos G Straten Particlevan Boxtel van (1999) ArticleTitleAn evolutionary strategy for fed-batch reactor optimization: concept and performance J. Biotechnol. 67 173–187
R Simutis A Lubbert (1997) ArticleTitleExploratory analysis of bioprocesses using artificial neural network based methods J. Biotechnol. 13 479–487
A Steinbuchel (1996) PHB and other polyhydroxyalkanoic acids H-J Rehm G Reed (Eds) Biotechnology. Weinheim VCH 403–464
Y Tian J Zhang AJ Morris (2002) ArticleTitleOptimal control of a fed-batch bioreactor based upon an augmented recurrent neural network model Neurocomputing 48 919–926
L Valdez-Castro I Baruch J Barrera-Cortes (2003) ArticleTitleNeural networks applied to the prediction of fed-batch fermentation kinetics of Bacillus thuringiensis Bioproc. Biosyst. Eng. 25 229–233
F Wang SY Lee (1997) ArticleTitlePoly(3-hydroxybutyrate) production with high productivity and high polymer content by a fed-batch culture of Alcaligenes eutrophus under nitrogen limitation Appl. Environ. Microbiol. 63 3703–3706
K Ye S Jin K Shimizu (1994) ArticleTitleFuzzy neural network for the control of high cell density cultivation of recombinant Escherichia coli J. Ferment. Bioeng. 77 663–673
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Patnaik, P.R. Neural network designs for poly- β-hydroxybutyrate production optimization under simulated industrial conditions. Biotechnol Lett 27, 409–415 (2005). https://doi.org/10.1007/s10529-005-1775-0
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DOI: https://doi.org/10.1007/s10529-005-1775-0