Abstract
The proanthocyanidin (PA) content was increased in seeds of pap1-D mutant of Arabidopsis thaliana, in which the expression of endogenous PAP1 gene encoding a Myb-like transcription factor was induced by activation-tagging with enhancer sequences derived from cauliflower mosaic virus 35S promoter. In contrast, the PA contents decreased in seeds of transgenic plants transformed with a PAP1 cDNA or with a PAP1 chimeric repressor, although the amount of soluble anthocyanins increased in seeds of transgenic plants over-expressing PAP1cDNA. The enhanced radical scavenging activity was observed only in the seed extracts of pap1-D mutant, indicating that PAs are primarily responsible for radical scavenging activity in seeds. The present study suggests the feasibility of engineering a transcription factor of flavonoid biosynthesis for health beneficial plant seeds.
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References
S Abrahams GJ Tanner PJ Larkin AR Ashton (2002) ArticleTitleIdentification and biochemical characterization of mutants in the proanthocyanidin pathway in Arabidopsis Plant Physiol. 130 561–576
BN Ames (1983) ArticleTitleDietary carcinogens and anticarcinogens. (oxygen radicals and degenerative diseases) Science 221 1256–1264
D Bagchi M Bagchi SJ Stohs DK Das SD Ray CA Kuszynski SS Joshi HG Pruess (2000) ArticleTitleFree radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention Toxicology 148 187–197
CW Beninger GL Hosfield (2003) ArticleTitleAntioxidant activity of extracts, condensed tannin fractions, and pure flavonoids from Phaseolus vulgaris L. seed coat color genotypes J. Agric. Food Chem. 51 7879–7883
MS Blois (1958) ArticleTitleAntioxidant determinations by the use of a stable free radical Nature 4617 1199–1200
SJ Bloor S Abrahams (2002) ArticleTitleThe structure of the major anthocyanin in Arabidopsis thaliana Phytochemistry 59 343–346
JO Borevitz Y Xia J Blount RA Dixon Lamb C (2000) ArticleTitleActivation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis Plant Cell 12 2383–2393
P Cos T De Bruyne N Hermans S Apers DV Berghe AJ Vlietinck (2004) ArticleTitleProanthocyanidins in health care: current and new trends Curr. Med. Chem. 11 1345–1359
SA Dalzell GL Kerven (1998) ArticleTitleA rapid method for the measurement of Leucaenaspp. proanthocyanidins by the proanthocyanidin (BuOH/HCl) assay J. Sci. Food Agric. 78 405–416
B Gabetta N Fuzzati A Griffini E Lolla R Pace T Ruffilli F Peterlongo (2000) ArticleTitleCharacterization of proanthocyanidins from grape seeds Fitoterapia 71 162–175
K Hiratsu K Matsui T Koyama M Ohme-Takagi (2003) ArticleTitleDominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in Arabidopsis Plant J. 34 733–739
DX Hou (2003) ArticleTitlePotential mechanisms of cancer chemoprevention by anthocyanins Curr. Mol. Med. 3 149–159
P Jones B Messner J Nakajima AR Schäffner Saito K (2003) ArticleTitleUGT73C6 and UGT78D1, glycosyltransferases involved in flavonol glycoside biosynthesis in Arabidopsis thaliana J. Biol. Chem. 45 43910–43918
LC Lin YC Kuo CJ Chou (2002) ArticleTitleImmunomodulatory proanthocyanidins from Ecdysanthera utilis J Nat Prod. 65 505–508
MAS Marles H Ray MY Gruber (2003) ArticleTitleNew perspectives on proanthocyanidin biochemistry and molecular regulation Phytochemistry 64 367–383
K Matsui H Tanaka M Ohme-takagi (2004) ArticleTitleSuppression of the biosynthesis of proanthocyanidin in Arabidopsis by a chimeric PAP1 repressor Plant Biotech. J. 2 487–493
J Nakajima I Tanaka S Seo M Yamazaki K Saito (2004) ArticleTitleLC/PDA/ESI-MS profiling and radical scavenging activity of anthocyanins in various berries J. Biomed. Biotech. 2004 241–247
R Niggeweg AJ Michael C Martin (2004) ArticleTitleEngineering plants with increased levels of the antioxidant chlorogenic acid Nature Biotech. 22 746–754
GW Plumb S De Pascual-Teresa C Santos-Buelga V Cheynier G Williamson (1998) ArticleTitleAntioxidant properties of catechins and proanthocyanidins: effect of polymerisation, galloylation and glycosylation Free Radic. Res. 129 351–358
H Ray M Yu P Auser L Blahut-Beatty B McKersie S Bowley N Westcott B Coulman A Llyod M Gruber (2003) ArticleTitleExpression of anthocyanins and proanthocyanidins after transformation of Alfalfa with Maize Lc Plant Physiol. 132 1448–1463
Y Takahata M Ohnishi-Kameyama S Furuta M Takahashi I Suda (2001) ArticleTitleHighly polymerized proanthocyanidins in brown soybean seed coat with a high radical-scavenging activity J. Agric. Food Chem. 49 5843–5847
Tohge T, Nishiyama Y, Yokota-Hirai M, Yano M, Nakajima J, Awazuhara M, Inoue E, Takahashi H, Goodenowe DB, Kitayama M, Noji M, Yamazaki M, Saito K. (2005) Functional genomics by integrated analysis of metabolome and transcriptome ofArabidopsis plants overexpressing a MYB transcription factor.Plant J. (in press)
M Veit GF Pauli (1999) ArticleTitleMajor flavonoids from Arabidopsis thaliana leaves J. Nat. Prod. 62 1301–1303
B Winkel-Shirley (2001) ArticleTitleIt takes a garden. How work on diverse plant species has contributed to an understanding of flavonoid metabolism Plant Physiol. 127 1399–1404
M Yamazaki J Nakajima M Yamanashi M Sugiyama Y Makita K Springob M Awazuhara K Saito (2003) ArticleTitleMetabolomics and differential gene expression in anthocyanin chemo-varietal forms of Perilla frutescens Phytochemistry 62 987–995
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Tohge, T., Matsui, K., Ohme-Takagi, M. et al. Enhanced radical scavenging activity of genetically modified Arabidopsis seeds. Biotechnol Lett 27, 297–303 (2005). https://doi.org/10.1007/s10529-005-0683-7
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DOI: https://doi.org/10.1007/s10529-005-0683-7