Skip to main content
SpringerLink
Log in

Enhanced neurite outgrowth of rat neural cortical cells on surface-modified films of poly(lactic-co-glycolic acid)

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Neural cortical cells, isolated from prenatal rat cerebra, were grown on surface-modified poly(lactic-co-glycolic acid, 65:35) (PLGA) films coated with poly-D-lysine (PDL) with either laminin (LN), fibronectin (FN) or collagen (CN). Immunocytochemistry showed that the isolated cells were highly immunopositive for both neurofilament and MAP-2 with well-organized neurites and somatodendritic localization. The presence of PDL with LN or FN on the PLGA films was essential for increased neural cell growth. Also, PLGA films coated with either PDL/LN or PDL/FN mixtures had higher neurite outgrowth and regular differentiation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • SB Bailey ME Eichler A Villadiego KM Rich (1993) ArticleTitleThe influence of fibronectin and laminin during Schwann cell migration and peripheral nerve regeneration through silicon chambers J. Neurocytol. 22 176–184

    Google Scholar 

  • AP Balgude X Yu A Szymanski RV Bellamkonda (2001) ArticleTitleAgarose gel stiffness determines rate of DRG neurite extension in 3D cultures Biomaterials 22 1077–1084

    Google Scholar 

  • S Blacher V Maquet F Schils D Martin J Schoenen G Moonen R Jerome J-P Pirard (2003) ArticleTitleImage analysis of the axonal ingrowth into poly(D,l-lactide) porous scaffolds in relation to the 3-D porous structures Biomaterials 24 1033–1040

    Google Scholar 

  • A Caceres G Banker L Binder (1986) ArticleTitleImmunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture J. Neurosci. 6 714–722

    Google Scholar 

  • CF Chu A Lu M Liszkowski R Sipehia (1999) ArticleTitleEnhanced growth of animal and human endothelial cells on biodegradable polymers Biochim. Biophys. Acta 1472 479–485

    Google Scholar 

  • J-L Dewez A Doren Y-J Schneider PG Rouxhet (1999) ArticleTitleCompetitive adsorption of proteins: key of the relationship between substratum surface properties and adhesion of epithelial cell Biomaterials 20 549–559

    Google Scholar 

  • YW Fan FZ Cui SP Hou QY Xu LN Chen I-S Lee (2002) ArticleTitleCulture of neural cells on silicon wafers with nano-scale surface topograph J. Neurosci. Meth. 120 17–23

    Google Scholar 

  • EL George EN Georges-Labouesse RS Patel-King H Rayburn RO Hynes (1993) ArticleTitleDefects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin Development 119 1079–1091

    Google Scholar 

  • Y Kanemura H Mori S Kobayashi O Islam E Kodama A Yamamoto Y Nakanishi N Arita M Yamasaki H Okano M Hara J Miyake (2002) ArticleTitleEvaluation of in vitro proliferative activity of human fetal neural stem/progenitor cells using indirect measurements of viable cells based on cellular metabolic activity J. Neurosci. Res. 69 869–879

    Google Scholar 

  • RP Lanzer R Langer WL Chick (1997) Principles of Tissue Engineering Landes Bioscience New York

    Google Scholar 

  • KY Lee DJ Mooney (2001) ArticleTitleHydrogels for tissue engineering Chem. Rev. 101 1869–1879

    Google Scholar 

  • AI Millaruelo M Nieto-Sampedro CW Cotman (1988) ArticleTitleCooperation between nerve growth factor and laminin or fibronectin in promoting sensory neuron survival and neurite outgrowth Brain Res. 466 219–228

    Google Scholar 

  • SM O’Connor DA Stenger KM Shaffer W Ma (2001) ArticleTitleSurvival and neurite outgrowth of rat cortical neurons in three-dimensional agarose and collagen gel matrices Neurosci. Lett. 304 189–193

    Google Scholar 

  • TJ O’Shaughnessy HJ Lin W Ma (2003) ArticleTitleFunctional synapse formation among rat cortical neurons grown on three-dimensional collagen gels Neurosci. Lett. 340 169–172

    Google Scholar 

  • KI Park YD Teng EY Snyder (2002) ArticleTitleThe injured brain interacts reciprocally with neural stem cells supported by scaffolds to reconstitute lost tissue Nat. Biotech. 20 1111–1117

    Google Scholar 

  • GE Rutkowski CA Heath (2002) ArticleTitleDevelopment of a bioartificial nerve graft II. Nerve regeneration in vitro. Biotechnol. Prog. 18 373–379

    Google Scholar 

  • S Sauberlich D Klee E-J Richter H Hocker H Spikermann (1999) ArticleTitleCell culture tests for assessing the tolerance of soft tissue to variously modified titanium surfaces Clin. Oral Implant Res. 10 379–393

    Google Scholar 

  • DA Stenger JJ Hickman KE Bateman MS Ravenscroft W Ma JJ Pancrazio K Shaffer AE Schaffner DH Cribbs CW Cotman (1998) ArticleTitleMicrolithographic determination of axonal/dendritic polarity in cultured hippocampal neurons J. Neurosci. Meth. 82 167–173

    Google Scholar 

  • MC Tate DA Shear SW Hoffman DG Stein DR Archer MC LaPlaca (2002) ArticleTitleFibronectin promotes survival and migration of primary neural stem cells transplanted into the traumatically injured mouse brain Cell Transplant. 11 283–295

    Google Scholar 

  • G Vozzi C Flaim A Ahluwalia S Bhatia (2003) ArticleTitleFabrication of PLGA scaffolds using soft lithography and microsyringe deposition Biomaterials 24 2533–2540

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong,Seodaemun-gu, 120-752, Seoul, Korea

    Dong-Wook Han, Min Sub Lee, Bong Joo Park & Jong-Chul Park

  2. Department of Biomedical EngineeringCollege of Biomedical Science and Engineering, Inje University, Kimhae, 621-749, Korea

    Jeong Koo Kim

  3. Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, 120-752, Korea

    Jong-Chul Park

Authors
  1. Dong-Wook Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Min Sub Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bong Joo Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jeong Koo Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jong-Chul Park
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jong-Chul Park.

Additional information

Revisions requested 30 September 2004; Revisions received 10 November 2004

Rights and permissions

Reprints and permissions

About this article

Cite this article

Han, DW., Sub Lee, M., Park, B.J. et al. Enhanced neurite outgrowth of rat neural cortical cells on surface-modified films of poly(lactic-co-glycolic acid). Biotechnol Lett 27, 53–58 (2005). https://doi.org/10.1007/s10529-004-6585-2

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-004-6585-2

Keywords

Search

Navigation