Abstract
The arachidonate cascade is important for the generation of reactive species (RS), and cyclooxygenase (COX) is a key enzyme of this cascade. Tissues of 24-month-old rat lung showed a 2-fold increase in RS, malondialdehyde and thromboxane B2 than those of 6-month-old rat. We found that the effects of 50 μM H2O2 and 200 μM t-butylhydroperoxide (t-BHP) specify on COX activity, and that their effects increased cytosolic COX activity in a concentration-dependent manner (1-50 μM) in 24-month-old rat. Our results suggested that COX activators such as t-BHP and H2O2, which are located in cytosol, are essential for the activation of COX in aged lung.
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ES Bos AA Doelen ParticleVan der N Rooy ParticleVan AH Schuurs (1981) ArticleTitle3,3′,5,5′-tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme immunoassay J. Immunoassay 2 187–204
DS Byun JT Venkatraman BP Yu G Fernandes (1995) ArticleTitleModulation of antioxidant activities and immune response by food restriction in aging Fisher-344 rats Aging Clin. Exp. Res. 7 40–48
HJ Fahn LS Wang RH Hsieh SC Chang SH Kao MH Huang YH Wei (1996) ArticleTitleAge-related 4,977 bp deletion in human lung mitochondrial DNA Am. J. Respir. Crit. Care Med. 154 1141–1145
D Harman (1992) ArticleTitleFree radical theory of aging Mutat. Res. 275 257–266
LJ Marnett P Wlodawer B Samuelsson (1975) ArticleTitleCooxygenation of organic substrates by the prostaglandin synthetase of sheep vesicular gland J. Biol. Chem. 250 8510–8517
J Mitchell H Jiang L Berry B Meyrick (1996) ArticleTitleEffect of antioxidants on lipopolysaccharide-stimulated induction of␣mangano superoxide dismutase mRNA in bovine pulmonary artery endothelial cells J. Cell Physiol. 169 333–340
G O’Neill AF Hutchinson (1993) ArticleTitleExpression of mRNA for cyclooxygenase-1 and cyclooxygenase-2 in human tissues FEBS Lett. 330 156–160
H Sheng J Shao EB Hooton M Tsujii RN DuBois RD Beauchamp (1997) ArticleTitleCyclooxygenase-2 induction and transforming growth factor beta growth inhibition in rat intestinal epithelial cells Cell Growth Differ. 8 463–470
V Vallyathan X Shi (1997) ArticleTitleThe role of oxygen free radicals in occupational and environmental lung diseases Environ. Health Perspect. 105 165–177
CS Williams RN DuBois (1996) ArticleTitleProstaglandin endoperoxide synthase: why two isoforms? Am J. Physiol. 270 G93–G400
BP Yu (1996) ArticleTitleAging and oxidative stress: modulation by dietary restriction Free Rad. Biol. Med. 21 651–668
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Im, J.W., Kim, N.D., Kim, N.D. et al. Activation of cyclooxygenases by H2O2and t-butylhydroperoxide in aged rat lung. Biotechnol Lett 26, 1665–1669 (2004). https://doi.org/10.1007/s10529-004-3519-y
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DOI: https://doi.org/10.1007/s10529-004-3519-y