Abstract
Different studies have shown that −238 G>A polymorphism in promoter region of tumor necrosis factor alpha (TNF-α) gene is associated with increased risk of non-alcoholic fatty liver disease (NAFLD). The current study investigates the association between metabolic parameters and nutritional intakes with −238 G>A of TNF-α promoter gene polymorphism among the Iranian patients with NAFLD. In this study, 75 patients with NAFLD and 76 individuals as control were enrolled. Dietary intakes were assessed using a semi-quantitative food-frequency questionnaire. Body mass index and waist to hip ratio were calculated. Biochemical assays were measured after 12 h fasting. −238 G>A Polymorphism of TNF-α gene was determined by using sequencing method. We observed no significant difference in frequency of different genotypes of this polymorphism between NAFLD and control groups (P > 0.05). Among biochemical parameters, TAC showed significant decrease in NAFLD patients with GG genotype when compared to controls (P = 0.001). The comparison of macro and micronutrient intakes between groups according to genotypes showed no statistically significant difference (P > 0.05). Although the data were not statistically significant, further studies with larger sample size are needed to determine the effect of dietary compounds in NAFLD.
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Acknowledgments
We appreciate all of the participants in the current study. We also thank Nutrition Research Center of Tabriz University of Medical Sciences and Cellular and Molecular Research Center of Qazvin University of Medical Science for providing laboratory facilities.
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This research was supported by a grant from cellular and molecular research center of Qazvin University of Medical Sciences.
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Mohseni, F., Rashvand, Z., Najafipour, R. et al. Evaluating −238 G>A Polymorphism Association in TNF-α Gene with Metabolic Parameters and Nutritional Intakes Among the Iranian NAFLD Patients. Biochem Genet 54, 685–695 (2016). https://doi.org/10.1007/s10528-016-9747-8
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DOI: https://doi.org/10.1007/s10528-016-9747-8