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Sequence of the Pearl Oyster Carbonic Anhydrase-Related Protein and Its Evolutionary Implications

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Abstract

Carbonic anhydrases are conserved in vertebrates and invertebrates, and a noncatalytic carbonic anhydrase-related protein VIII (CARP VIII) has been found in deuterostomes and the phylum Placozoa. I isolated a cDNA encoding a noncatalytic CARP from the mantle of the pearl oyster Pinctada fucata. The polypeptide (CARP-1) predicted from the nucleotide sequence shares 44–60% identity with known CARP VIII sequences, and its phylogenetic analysis showed that P. fucata formed a single group with deuterostome invertebrates. However, since CARP VIII sequences are not identified in protostomes, these results suggest that CARP-1 may have originated in molluscs independently from deuterostome CARP VIII sequences.

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Acknowledgments

This study was supported by a grant from Wakayama Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence of JST (Japan Science and Technology Agency).

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  1. Department of Genetic Engineering, Kinki University, 930 Nishimitani, Kinokawa, Wakayama, 649-6493, Japan

    Hiroshi Miyamoto

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  1. Hiroshi Miyamoto
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Correspondence to Hiroshi Miyamoto.

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Miyamoto, H. Sequence of the Pearl Oyster Carbonic Anhydrase-Related Protein and Its Evolutionary Implications. Biochem Genet 50, 269–276 (2012). https://doi.org/10.1007/s10528-011-9469-x

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