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Association of B3GNT5 Polymorphisms with Susceptibility to ETEC F4ab/ac in the White Duroc × Erhualian Intercross and 15 Outbred Pig Breeds

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Abstract

The B3GNT5 gene is a candidate for the F4ab/ac receptor conferring susceptibility to enterotoxigenic Escherichia coli (ETEC) F4ab/ac in pigs. In this study, we screened mutations in the complete coding region of the porcine B3GNT5 gene and identified four SNPs in the 3′ untranslated regions. We genotyped the four SNPs across a large-scale White Duroc × Chinese Erhualian F 2 resource population (total F 2 = 755) and 292 purebred piglets representing 15 Chinese and Western breeds. We found that the g.1476G→A locus and haplotypes [A;T;G;T] and [A;G;G;T] had significant association with susceptibility to ETEC F4ac in the resource population. None of the B3GNT5 polymorphisms and haplotypes was associated with susceptibility to ETEC F4ab/ac in outbred piglets. This result, together with other reports, supports the conclusion that B3GNT5 is not the responsible gene encoding the ETEC F4ab/ac receptors.

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Acknowledgments

This study was supported by the National 863 Project of China (2006AA10Z194) and the Jiangxi Provincial Key Research Project.

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Authors and Affiliations

  1. Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, Nanchang, 330045, China

    Jing Ouyang, Weihong Zeng, Jun Ren, Xueming Yan, Zhiyan Zhang, Ming Yang, Pengfei Han, Xiang Huang, Huashui Ai & Lusheng Huang

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  1. Jing Ouyang
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  2. Weihong Zeng
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  3. Jun Ren
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  4. Xueming Yan
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  5. Zhiyan Zhang
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  6. Ming Yang
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  7. Pengfei Han
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  8. Xiang Huang
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  9. Huashui Ai
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  10. Lusheng Huang
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Corresponding author

Correspondence to Lusheng Huang.

Additional information

Jing Ouyang and Weihong Zeng contributed equally to this study.

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Ouyang, J., Zeng, W., Ren, J. et al. Association of B3GNT5 Polymorphisms with Susceptibility to ETEC F4ab/ac in the White Duroc × Erhualian Intercross and 15 Outbred Pig Breeds. Biochem Genet 50, 19–33 (2012). https://doi.org/10.1007/s10528-011-9454-4

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  • DOI: https://doi.org/10.1007/s10528-011-9454-4

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