Skip to main content

Advertisement

SpringerLink
Log in

Haplotypes in the Interleukin 8 Gene and Their Association with Chronic Periodontitis Susceptibility

  • Published:
Biochemical Genetics Aims and scope Submit manuscript

Abstract

Interleukin-8 (IL-8), which is responsible for the migration and activation of neutrophils, is an important inflammatory mediator involved in the initiation and amplification of acute inflammatory reactions and chronic inflammatory processes. IL-8 plays an important role in periodontitis, an inflammatory disease characterized by the loss of connective tissue and alveolar bone. The aim of this study was to investigate whether the SNPs rs2227307 (+396) and rs2227306 (+781), and the haplotypes they formed together with the previously investigated rs4073 (−251), were associated with chronic periodontitis susceptibility. Clinical periodontal exams were performed and DNA samples were collected from 493 individuals (223 with periodontitis and 270 controls). Associations between SNPs, haplotypes, and subject phenotypes were analyzed using the χ2 test followed by multivariate logistic regression modeling. We conclude that the +396TT genotype and the haplotypes ATC/TTC and AGT/TGC were significantly associated with chronic periodontitis susceptibility in Brazilians.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Brett PM, Zygogianni P, Griffiths GS, Tomaz M, Parkar M, D’Aiuto F, Tonetti M (2005) Functional gene polymorphisms in aggressive and chronic periodontitis. J Dent Res 84:1149–1153

    Article  PubMed  CAS  Google Scholar 

  • Brown EE, Fallin D, Ruczinski I, Hutchinson A, Staats B, Vitale F, Lauria C, Serraino D, Rezza G, Mbisa G, Whitby D, Messina A, Goedert JJ, Chanock SJ (2006) Associations of classic Kaposi sarcoma with common variants in genes that modulate host immunity. Cancer Epidemiol Biomarkers Prev 15:926–934

    Article  PubMed  CAS  Google Scholar 

  • Callegari-Jacques SM, Salzano FM (1999) Brazilian Indian/non-Indian interactions and their effects. Cienc Cult 51:166–174

    Google Scholar 

  • Campa D, Hung RJ, Mates D, Zaridzi D, Szeszenia-Dabrouska N, Rudnai P, Lissouska J, Fabiánova E, Bencko V, Foretova L (2005) Lack of association between −251 T > A polymorphism of IL8 and lung cancer risk. Cancer Epidemiol Biomarkers Prev 14:2457–2458

    Article  PubMed  CAS  Google Scholar 

  • De Brito RB Jr, Scarel-Caminaga RM, Trevilatto PC, Souza AP, Barros SP (2004) Polymorphisms in the vitamin D receptor gene are associated with periodontal disease. J Periodontol 75:1090–1095

    Article  Google Scholar 

  • Dini EL, Castellanos RA (1995) CPITN: time and cost estimates for periodontal prevention and treatment procedures. Braz Dent J 6:53–58

    PubMed  CAS  Google Scholar 

  • Dongari-Bagtzoglou AI, Ebersole JL (1998) Increased presence of interleukin-6 (IL-6) and IL-8 secreting fibroblast subpopulations in adult periodontitis. J Periodontol 69:899–910

    PubMed  CAS  Google Scholar 

  • Hacking D, Knight JC, Rockett K, Brown H, Frampton J, Kwiatkowski DP, Hull J, Udalova IA (2004) Increased in vivo transcription of an IL-8 haplotype associated with respiratory syncytial virus disease-susceptibility. Genes Immun 5:274–282

    Article  PubMed  CAS  Google Scholar 

  • Hull J, Thomson A, Kwiatkowski D (2000) Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families. Thorax 55:1023–1027

    Article  PubMed  CAS  Google Scholar 

  • Hull J, Ackerman H, Isles K, Usen S, Pinder M, Thonsom A, Kwiatkowski D (2001) Unusual haplotypic structure of IL8, a susceptibility locus for a common respiratory virus. Am J Hum Genet 69:413–419

    Article  PubMed  CAS  Google Scholar 

  • Ito H, Matsuo K, Hamajima N, Okuma K, Saito T, Tajima K (2005) Significant association of interleukin 8 −251T/A polymorphism with smoking behavior in a Japanese population. J Hum Genet 11:567–573

    Article  Google Scholar 

  • Kamali-Sarvestani E, Nikseresht AR, Aliparasti MR, Vessal M (2006) IL-8 (−251 A/T) and CXCR2 (+1208 C/T) gene polymorphisms and risk of multiple sclerosis in Iranian patients. Neurosci Lett 404:159–162

    Article  PubMed  CAS  Google Scholar 

  • Kim YJ, Viana AC, Curtis KM, Orrico SR, Cirelli JA, Scarel-Caminaga RM (2009a) Lack of association of a functional polymorphism in the interleukin 8 gene with susceptibility to periodontitis. DNA Cell Biol 28:185–190

    Article  PubMed  CAS  Google Scholar 

  • Kim YJ, Viana AC, Corbi SCT, Curtis KMC, Renzi R, Orrico SRP, Cirelli JA, Scarel-Caminaga (2009b) Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a PCR–RFLP assay. Biosci J 25:134–142

    Google Scholar 

  • Kinane DF, Hodge P, Eskdale J, Ellis R, Gallagher G (1999) Analysis of genetic polymorphisms at the interleukin-10 and tumour necrosis factor loci in early-onset periodontitis. J Periodontal Res 34:379–386

    Article  PubMed  CAS  Google Scholar 

  • Kornman KS, Crane A, Wang HY, Di giovine FS, Newman MG, Pirk FW, Wilson TG Jr, Higginbottom FL, Duff GW (1997) The interleukin-1 genotype as a severity factor in adult periodontal disease. J Clin Periodontol 24:72–77

    Article  PubMed  CAS  Google Scholar 

  • Michalowicz BS (1994) Genetic and heritable risk factors in periodontal disease. J Periodontol 65:479–488

    PubMed  CAS  Google Scholar 

  • Michalowicz BS, Aeppli D, Virag JG, Klump DG, Hinrichs JE, Segal NL, Bouchard TJ Jr, Pihlstrom BL (1991) Periodontal findings in adults twins. J Periodontol 62:293–299

    PubMed  CAS  Google Scholar 

  • Moreira PR, Costa JE, Gomez RS, Gollob KJ, Dutra WO (2007) The IL1A (−889) gene polymorphism is associated with chronic periodontal disease in a sample of Brazilian individuals. J Periodontal Res 42:23–30

    Article  PubMed  CAS  Google Scholar 

  • Okada H, Murakami S (1998) Cytokine expression in periodontal health and disease. Crit Rev Oral Biol Med 9:248–266

    Article  PubMed  CAS  Google Scholar 

  • Page RC, Sims TJ, Engel LD, Moncla BJ, Bainbridge B, Stray J, Darveau RP (1991) The immunodominant outer membrane antigen of Actinobacillus actinomycetemcomitans is located in the serotype-specific high-molecular-mass carbohydrate moiety of lipopolysaccharide. Infect Immun 59:3451–3462

    PubMed  CAS  Google Scholar 

  • Park BL, Kim LH, Choi YH, Lee JH, Rhim T, Lee YM, Uh ST, Park HS, Choi BW, Hong SJ, Park CS, Shin HD (2004) Interleukin 3 (IL3) polymorphisms associated with decreased risk of asthma and atopy. J Hum Genet 49:517–527

    Article  PubMed  CAS  Google Scholar 

  • Peres MA, Antunes JL, Boing AF, Peres KG, Bastos JL (2007) Skin colour is associated with periodontal disease in Brazilian adults: a population-based oral health survey. J Clin Periodontol 34:196–201

    Article  PubMed  Google Scholar 

  • Purcell S, Chemy SS, Sham PC (2003) Genetic Power Calculator: design of linkage and association genetic mapping studies of complex traits. Bioinformatics 19:149–150

    Article  PubMed  CAS  Google Scholar 

  • Puthothu B, Krueger M, Heinze J, Foster J, Heinzmann A (2006) Impact of IL8 and IL8-receptor alpha polymorphisms on the genetics of bronchial asthma and severe RSV infections. Clin Mol Allergy 4:2

    Article  PubMed  Google Scholar 

  • Renzoni E, Lympany P, Sestini P, Panteldis P, Wells A, Black C, Welsh K, Bunn C, Knight C, Foley P (2000) Distribution of novel polymorphisms of the interleukin-8 and CXC receptor 1 and 2 genes in systemic sclerosis and cryptogenic fibrosing alveolitis. Arthritis Rheum 43:1633–1640

    Article  PubMed  CAS  Google Scholar 

  • Rovin BH, Lu L, Zhang X (2002) A novel interleukin-8 polymorphism is associated with severe systemic lupus erythematosus nephritis. Kidney Int 62:261–265

    Article  PubMed  CAS  Google Scholar 

  • Sanguinetti CJ, Dias-Neto E, Simpson AJ (1994) Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques 14:914–921

    Google Scholar 

  • Savage SA, Abnet CC, Mark SD, Qiao YL, Dong ZW, Dawsey SM, Taylor PR, Chanock SJ (2004) Variants of the IL8 and IL8RB genes and risk for gastric cardia adenocarcinoma and esophageal squamous cell carcinoma. Cancer Epidemiol Biomarkers Prev 13:2251–2257

    PubMed  CAS  Google Scholar 

  • Scarel-Caminaga RM, Trevilatto PC, Souza AP, Brito RB Jr, Line SRP (2002) Investigation of an IL-2 polymorphism in patients with different levels of chronic periodontitis. J Clin Periodontol 29:587–591

    Article  PubMed  CAS  Google Scholar 

  • Scarel-Caminaga RM, Trevilatto PC, Souza AP, Brito RB, Camargo LE, Line SR (2004) Interleukin 10 gene promoter polymorphism are associated with chronic periodontitis. J Clin Periodontol 31:443–448

    Article  PubMed  CAS  Google Scholar 

  • Sham PC, Curtis D (1995) Monte Carlo tests for associations between disease and alleles at highly polymorphic loci. Ann Hum Genet 59:97–105

    Article  PubMed  CAS  Google Scholar 

  • Socransky SS, Haffajee AD, Smith C, Duff GW (2000) Microbiological parameters associated with IL-1 gene polymorphisms in periodontitis patients. J Clin Periodontol 27:810–818

    Article  PubMed  CAS  Google Scholar 

  • Viana AC, Curtis KMC, Kim YJ, Orrico SRP, Cano VSP, Valentini SR, Scarel-Caminaga RM (2009) Amplification-created restriction site (ACRS) method for detection of the SNP rs2227306 in the interleukin 8 gene a interethnic comparison. SaudPesq 2:27–31

    Google Scholar 

Download references

Acknowledgments

This study was supported by São Paulo Research Foundation (FAPESP) grants 2003/10424-0, 2005/03231-7, and 2006/04492. The authors report no conflicts of interest related to this study.

Author information

Authors and Affiliations

  1. Department of Morphology, School of Dentistry at Araraquara, UNESP—São Paulo State University, CP. 331, Araraquara, SP, 14801-903, Brazil

    Raquel M. Scarel-Caminaga, Karen M. C. Curtis & Patrícia M. Sogumo

  2. Department of Oral Diagnosis and Surgery, School of Dentistry at Araraquara, UNESP—São Paulo State University, Araraquara, SP, Brazil

    Yeon J. Kim, Aline C. Viana, Sâmia C. T. Corbi, Silvana R. P. Orrico & Joni A. Cirelli

Authors
  1. Raquel M. Scarel-Caminaga
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yeon J. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aline C. Viana
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Karen M. C. Curtis
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sâmia C. T. Corbi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Patrícia M. Sogumo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Silvana R. P. Orrico
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Joni A. Cirelli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Raquel M. Scarel-Caminaga.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scarel-Caminaga, R.M., Kim, Y.J., Viana, A.C. et al. Haplotypes in the Interleukin 8 Gene and Their Association with Chronic Periodontitis Susceptibility. Biochem Genet 49, 292–302 (2011). https://doi.org/10.1007/s10528-010-9407-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10528-010-9407-3

Keywords

Search

Navigation