Abstract
In an analysis of the possible association of endothelial constitutive nitric oxide synthase (ecNOS) gene polymorphism and plasma nitric oxide levels in patients with acute coronary syndrome, we investigated 106 patients with the syndrome and 100 healthy controls. Genotype was determined using the polymerase chain reaction; plasma nitric oxide levels were found using ELISA. The genotype frequencies for the a/b polymorphism in the control group were 77% for bb, 19% for ab, and 4% for aa. In the patients, genotype frequencies were 55% bb, 34% ab, and 11% aa. The allele frequencies were 28% a and 72% b among the patients and 13% a and 87% b among control subjects. Our findings showed a significant association of the ecNOS gene polymorphism with acute coronary syndrome in the South Indian population.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Akar N, Akar E, Cin S, Deda G, Avcu F, Yalcin A (1999) Endothelial nitric oxide synthase intron 4, 27 bp repeat polymorphism in Turkish patients with deep vein thrombosis and cerebrovascular accidents. Thromb Res 94:63–64
Beckman J, Tsai JH (1994) Reactions and diffusion of nitric oxide and peroxynitrite. Biochemist 16:8–10
Cai H, Harrison DG (2000) Endothelial dysfunction in cardio vascular diseases. Circ Res 87:840–844
Gardemann A, Lohre J, Cayci S, Katz N, Tillmanns H, Haberbosch W (2002) The T allele of the missense Glu298Asp endothelial nitric oxide synthase gene polymorphism is associated with coronary heart disease in younger individuals with high atherosclerosis risk profile. Atherosclerosis 160:167–175
Graham A, Hogg N, Kalyanoraman B, Oleary V, Darley-Usmar V, Moncada S (1993) Peroxynitrite modifications of LDL lead to recognition by the macrophage scavenger platelets. FEBS Lett 330:181–185
Ichihara S, Yamada Y, Fujimura T, Nakashima N, Yokota M (1998) Association of a polymorphism of the endothelial constitutive nitric oxide synthase gene with myocardial infarction in the Japanese population. Am J Cardiol 18:83–86
Jackson R, Scragg R, Marshall R, White H, O’Brien K, Small C (1987) Changes in serum lipid concentrations during first 24 hours after myocardial infarction. Br Med J 294:1588–1589
Kugiyama Yasue H, Okumura K, Ogawa H, Fujimoto K, Nakao K, Yoshimura M, Motoyama T, Inobe Y, Kawano H (1996) Nitric oxide activity is deficient in spasm arteries of patients with coronary spastic angina. Circulation 24:266–272
Marsden PA, Shappert KT, Chen HS, Flowers M, Sundell CL, Wilcox JN, Lamas S, Michel T (1992) Molecular cloning and characterization of human endothelial nitric oxide synthase. FEBS Lett 307:287–293
Miller SA, Dykes DD, Polesky HF (1988) A simple salting out method for extracting DNA from human nucleated cells. Nucl Acids Res 16:12–15
Ordonez AJ, Carreira JM, Franco AG, Sanchez LM, Alvarez MV, Garcia EC (2000) Two expressive polymorphisms on the endothelial nitric oxide synthase gene (intron 4, 27 bp repeat and -786 T/C) and the venous thrombosis. Thromb Res 99:563–566
Park KW, You KH, Oh S, Chae IH, Kim HS, Oh BH, Lee MM, Park YB (2004) Association of endothelial constitutive nitric oxide synthase gene polymorphism with acute coronary syndrome in Koreans. Heart 90:282–285
Rao S, Austin H, Davidoff MN, Zafari AM (2005) Endothelial nitric oxide synthase intron 4 polymorphism is a marker for coronary artery disease in African-American and Caucasian men. Ethn Dis 15:191–197
Ryder REJ, Hayes TM, Mulligan IP, Kingswood JC, Williams S, Owens DR (1984) How soon after myocardial infarction should plasma lipid values be assessed? Br Med J 289:1651–1653
Salimi S, Firoozrai M, Nourmohammadi I, Shabani M, Mohebbi A (2006) Endothelial nitric oxide synthase gene intron 4 VNTR polymorphism in patients with coronary artery disease in Iran. Indian J Med Res 124:683–688
Sewdarsen M, Vythilingum S, Jialal I, Nadar R (1988) Plasma lipid can be reliably assessed within 24 hrs after acute myocardial infarction. Postgrad Medical J 64:352–356
Sharma SP (2004) Nitric oxide and the kidney. Indian J Nephrol 14:77–84
Sozmen B, Kazaz C, Taskiran D, L Aslan, Akyol A, Sozmen EY (1998) Plasma antioxidant status and nitrate levels with hypertension and coronary artery disease. Trurk J Med Sci 28:525–531
Tsimikas S, Bergmark C, Beyer RW, Patel R, Pattison J, Miller E, Juliano J, Witztum JL (2003) Temporal increases in plasma markers of oxidized low density lipoprotein strongly reflect the presence of acute coronary syndrome. J Am Coll Cardiol 41:360–370
Tsukada T, Yokohama K, Aria T, Takemoto F, Hara S, Yamada A, Kawaguchi Y, Hosoya T, Igari J (1998) Evidence of association of ecNOS gene polymorphism with plasma NO metabolic levels in humans. Biochem Biophys Res Commun 245:190–193
Turko IV, Murad F (2002) Protein nitration in cardiovascular diseases. Pharmacol Rev 54:619–634
Vallance P, Collier J, Moncada S (1989) Effects of endothelium-derived nitric oxide on peripheral arteriolar tone in man. Lancet 2:997–1000
Wang XL, Sim AS, Bandenhop RF, McCredie RM, Wilcken DE (1996) A smoking dependent risk of coronary artery disease associated with a polymorphism of endothelial nitric oxide synthase gene. Nat Med 2:41–45
Wang XL, Mahaney MC, Sim AS, Wang J, Wang J, Blangero J, Almasy L, Badenhop RB, Wilcken DE (1997) Genetic contribution of endothelial constitutive NO synthase gene to plasma nitric oxide levels. Artheroscler Thromb Vasc Biol 17:3147–3153
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gururajan, P., Gurumurthy, P., Victor, D. et al. Plasma Total Nitric Oxide and Endothelial Constitutive Nitric Oxide Synthase (ecNOS) Gene Polymorphism: A Study in a South Indian Population. Biochem Genet 49, 96–103 (2011). https://doi.org/10.1007/s10528-010-9391-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10528-010-9391-7