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Effect of Strontium Ranelate on Hydrogen Peroxide-Induced Apoptosis of CRL-11372 Cells

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Abstract

In vitro and in vivo studies have proven strontium to be an osteoinductive trace element. The effect of strontium ranelate (SR) on H2O2-induced apoptosis of CRL-11372 cells and optimization of its anti-apoptotic dose were the aims of this study. After 1 h of pretreatment with SR 1 μM, 50 μM, 100 μM, 500 μM, and 1,000 μM concentrations, CRL-11372 osteoblasts were exposed to 100 μM H2O2 for periods of 6–12 h. The same experiments were repeated without H2O2. The apoptotic index and viability of cells were assessed quantitatively with a fluorescent dye and qualitatively with agarose gel electrophoresis. Concentrations of 1–100 μM of SR with a 6-h treatment and only 1 μM concentration with a 12-h treatment inhibited the apoptotic effect of H2O2 on cultured osteoblasts significantly (P < 0.05). SR was shown to inhibit H2O2-induced apoptosis of CRL-11372 cells in a dose-dependent manner.

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Authors and Affiliations

  1. Department of Orthopaedics and Traumatology, Faculty of Medicine, Gazi University, Besevler, 06510, Ankara, Turkey

    Alpaslan Senkoylu, Aykin Simsek, Necdet Altun & Selcuk Bolukbasi

  2. Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, Besevler, 06510, Ankara, Turkey

    Akin Yilmaz & Sevda Menevse

  3. Department of Medical Genetics, Faculty of Medicine, Gazi University, Besevler, 06510, Ankara, Turkey

    Mehmet Ali Ergun

  4. Department of Public Health, Faculty of Medicine, Gazi University, Besevler, 06510, Ankara, Turkey

    Mustafa N. İlhan

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  1. Alpaslan Senkoylu
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  2. Akin Yilmaz
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  3. Mehmet Ali Ergun
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  4. Mustafa N. İlhan
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  5. Aykin Simsek
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Correspondence to Mehmet Ali Ergun.

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Senkoylu, A., Yilmaz, A., Ergun, M.A. et al. Effect of Strontium Ranelate on Hydrogen Peroxide-Induced Apoptosis of CRL-11372 Cells. Biochem Genet 46, 197–205 (2008). https://doi.org/10.1007/s10528-008-9142-1

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  • DOI: https://doi.org/10.1007/s10528-008-9142-1

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