We report the development and characterization of three new microsatellite markers in the tsetse fly, Glossina pallidipes (Diptera: Glossinidae). Fifty-eight alleles were scored in 192 individuals representing six natural populations. Allelic diversity ranged from 9 to 28 alleles per locus (mean 19.3 ± 5.5). Averaged across loci, observed heterozygosity was 0.581 ± 0.209, and expected heterozygosity was 0.619 ± 0.181. Cross-species amplifications of the G. pallidipes loci in other tsetse fly taxa are reported.
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This research was funded by USPHS-NIH grant AI-052456.
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Ouma, J.O., Marquez, J.G. & Krafsur, E.S. New Polymorphic Microsatellites in Glossina pallidipes (Diptera: Glossinidae) and Their Cross-Amplification in Other Tsetse Fly Taxa. Biochem Genet 44, 464–470 (2006). https://doi.org/10.1007/s10528-006-9046-x
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DOI: https://doi.org/10.1007/s10528-006-9046-x