A single amino acid substitution between Asn and Ser at position 631 in the chicken Mx protein has been reported to determine resistant and sensitive antiviral activity. In this study, we investigate whether various kinds of chicken breeds and jungle fowls carry the resistant or sensitive Mx allelic gene by using the mismatched PCR-restriction fragment length polymorphism (RFLP) technique. In total, 271 samples from 36 strains of 17 chicken breeds and from 3 kinds of jungle fowls were examined. The rates of the resistant Mx gene and sensitive gene were 59.2% and 40.8%, respectively. Only a Red jungle fowl captured in Laos carried the resistant Mx gene, and the other three Red jungle fowls from Indonesia and Gray and Green jungle fowls all had the sensitive Mx gene. These results were confirmed by the determination of amino acid sequences in the GTPase effector domain of jungle fowls.
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REFERENCES
Aebi, M., Fah, J., Hurt, N., Samuel, C. E., Thomis, D., Bazzigher, L., Pavlovic, J., Haller, O., and Staeheli, P. (1989). cDNA structures and regulation of two interferon-induced human Mx proteins. Mol. Cell. Biol. 9:5062–5072.
Bazzigher, L., Schwarz, A., and Staeheli, P. (1993). No enhanced influenza virus resistance of murine and avian cells expressing cloned duck Mx protein. Virology 195:100–112.
Bernasconi, D., Schultz, U., and Staeheli, P. (1995). The interferon-induced Mx protein of chickens lacks antiviral activity. J. Interferon Cytokine Res. 15:47–53.
Charleston, B., and Stewart, H. J. (1993). An interferon-induced Mx protein: cDNA sequence and high-level expression in the endometrium of pregnant sheep. Gene 137:327–331.
Easterday, B. C. (1975). Animal influenza. In Kilbourneed, E. D. (ed.), The Influenza Viruses and Influenza, Academic Press, New York, pp. 449–481.
Fumihito, A., Miyake, T., Sumi, S., Takada, M., Ohno, S., and Kondo, N. (1994). One subspecies of the red jungle fowl (Gallus gallus gallus) suffices as the matriarchic ancestor of all domestic breeds. Proc. Natl. Acad. Sci. U.S.A. 91:12505–12509.
Fumihito, A., Miyake, T., Takada, M., Shingu, R., Endo, T., Gojobori, T., Kondo, N., and Ohno, S. (1996). Monophyletic origin and unique dispersal patterns of domestic fowls. Proc. Natl. Acad. Sci. U.S.A. 93:6792–6795.
Haller, O., Acklin, M., and Staeheli, P. (1987). Influenza virus resistance of wild mice: Wild-type and mutant Mx alleles occur at comparable frequencies. J. Interferon Cytokine Res. 7:647–656.
Haller, O., Frese, M., Rost, D., Nuttall, P. A., and Kochs, G. (1995). Tick-borne thogoto virus infection in mice is inhibited by the orthomyxovirus resistance gene product Mx1. J. Virol. 69:2596–2601.
Haller, O., and Kochs, G. (2002). Interferon-induced Mx proteins: Dynamin-like GTPases with antiviral activity. Traffic 3:710–717.
Horisberger, M. A. (1992). Interferon-induced human protein MxA is a GTPase which binds transiently to cellular proteins. J. Virol. 66:4705–4709.
Ko, J. H., Jin, H. K., Asano, A., Takada, A., Ninomiya, A., Kida, H., Hokiyama, H., Ohara, M., Tsuzuki, M., Nishibori, M., Mizutani, M., and Watanabe, T. (2002). Polymorphisms and the differential antiviral activity of the chicken Mx gene. Genome Res. 12:595–601.
Ko, J. H., Takada, A., Mitsuhashi, T., Agui, T., and Watanabe, T. (2004). Native antiviral specificity of chicken Mx protein depends on amino acid variation at position 631. Anim. Genet. 35:119–122.
Lee, S. H., and Vidal, S. M. (2002). Functional diversity of Mx proteins: Variations on a theme of host resistance to infection. Genome Res. 12:527–530.
Meier, E., Kunz, G., Haller, O., and Arnheiter, H. (1990). Activity of rat Mx proteins against a rhabdovirus. J. Virol. 64: 6263–9.
Müller, M., Winnacker, E. L., and Brem, G. (1992). Molecular cloning of porcine Mx cDNAs: New members of a family of interferon-inducible proteins with homology to GTP-binding proteins. J. Interferon Cytokine Res. 12:119–129.
Nakayama, M., Nagata, K., and Ishihama, A. (1992). Enzymatic properties of the mouse Mx1 protein-associated GTPase. Virus Res. 22:227–234.
Nakayama, M., Nagata, K., Kato, A., and Ishihama, A. (1991). Interferon-inducible mouse Mx1 protein that confers resistance to influenza virus is GTPase. J. Biol. Chem. 266:21404–21408.
Nakayama, M., Yazaki, K., Kusano, A., Nagata, K., Hanai, N., and Ishihama, A. (1993). Structure of mouse Mx1 protein: Molecular assembly and GTP-dependent conformational change. J. Biol. Chem. 268:15033–15038.
Pavlovic, J., Zurcher, T., Haller, O., and Staeheli, P. (1990). Resistance to influenza virus and vesicular stomatitis virus conferred by expression of human MxA protein. J. Virol. 64:3370–3375.
Pitossi, F., Blank, A., Schroder, A., Schwarz, A., Hussi, P., Schwemmle, M., Pavlovic, J., and Staeheli, P. (1993). A functional GTP-binding motif is necessary for antiviral activity of Mx proteins. J. Virol. 67:6726–6732.
Richter, M. F., Schwemmle, M., Herrmann, C., Wittinghofer, A., and Staeheli, P. (1995). Interferon-induced MxA protein. GTP binding and GTP hydrolysis properties. J. Biol. Chem. 270:13512–13517.
Samuel, C. E. (1991). Antiviral actions of interferon: Interferon-regulated cellular proteins and their surprisingly selective antiviral activities. Virology 183:1–11.
Schnorr, J. J., Schneider-Schaulies, S., Simon-Jodicke, A., Pavlovic, J., Horisberger, M. A., and Meulen, V. (1993). MxA-dependent inhibition of measles virus glycoprotein synthesis in a stably transfected human monocytic cell line. J. Virol. 67:4760–4768.
Staeheli, P. (1990). Interferon-induced proteins and the antiviral state. Adv. Virus Res. 38:147–200.
Subbarao, K., Klimov, A., Katz, J., Regnery, H., Lim, W., Hall, H., Perdue, M., Swayne, D., Bender, C., Huang, J., Hemphill, M., Rowe, T., Shaw, M., Xu, X., Fukuda, K., and Cox, N. (1998). Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness. Science 279:393–396.
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Seyama, T., Ko, J.H., Ohe, M. et al. Population Research of Genetic Polymorphism at Amino Acid Position 631 in Chicken Mx Protein with Differential Antiviral Activity. Biochem Genet 44, 432–443 (2006). https://doi.org/10.1007/s10528-006-9040-3
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DOI: https://doi.org/10.1007/s10528-006-9040-3