Abstract
Parasitoid wasps have received a great deal of attention in the biological control of melon-cotton aphid (Aphis gossypii Glover). The species of parasitoids are often difficult to identify because of their small body size and profound diversity. DNA barcoding offers scientists who are not expert taxonomists a powerful tool to render their field studies more accurate. Using DNA barcodes to identify aphid parasitoid wasps in specific cropping systems may provide valuable information for biological control. Here, we report the use of DNA barcoding to confirm the morphological identification of 14 species (belonging to 13 genera of 7 families) of parasitoid wasps from two-year field samples in a watermelon cropping system. We generated DNA sequences from the mitochondrial COI gene and the nuclear D2 region of 28S rDNA to assess the genetic variation within and between parasitoid species. Automatic Barcode Gap Discovery (ABGD) supported the presence of 14 genetically distinct groups in the dataset. Among the COI sequences, we found no overlap between the maximum K2P distance within species (0.49%) and minimum distance between species (6.85%). The 28S sequences also showed greater interspecific distance than intraspecific distance. DNA barcoding confirmed the morphological identification. However, inconsistency and ambiguity of taxonomic information available in the online databases has limited the successful use of DNA barcoding. Only five species matched those in the BOLD and GenBank. Four species did not match the entries in GenBank and five species showed ambiguous results in BOLD due to confusing nomenclature. We suggested that species identification based on DNA barcodes should be performed using both COI and other genes. Nonetheless, we demonstrate the potential of the DNA barcoding approach to confirm field identifications and to provide a foundation for studies aimed at improving the understanding of the biocontrol services provided by parasitoids in the melon ecosystem.
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Acknowledgements
We would like to thank Yifan Li, Yuansheng Li, and Baofeng Mu for providing assistance with sampling and DNA extraction and the farmers for allowing us to collect aphids from their fields. We also thank Dr. Xu Zhang (Institute of Zoology, Chinese Academy of Sciences) for assistance in parasitoid identification. This study was supported by National Key R&D Program of China (2017YFD0200300), National Natural Science Foundation of China (No. 31501646) and Scientific Research Project of Beijing Municipal Education Commission (KM201710020006).
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Dong, Z., Liu, S. & Zhang, Z. Efficacy of using DNA barcoding to identify parasitoid wasps of the melon-cotton aphid (Aphis gossypii) in watermelon cropping system. BioControl 63, 677–685 (2018). https://doi.org/10.1007/s10526-018-9894-4
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DOI: https://doi.org/10.1007/s10526-018-9894-4