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Induction of pathogenesis-related proteins during biocontrol of Rhizoctonia solani with Pseudomonas aureofaciens in soybean (Glycine max L. Merr.) plants

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Abstract

To investigate the biocontrol effectiveness of the antibiotic producing bacterium, Pseudomonas aureofaciens 63–28 against the phytopathogen Rhizoctonia solani AG-4 on Petri plates and in soybean roots, growth response and induction of PR-proteins were estimated after inoculation with P. aureofaciens 63–28 (P), with R. solani AG-4 (R), or with P. aureofaciens 63–28 + R. solani AG-4 (P + R). P. aureofaciens 63–28 showed strong antifungal activity against R. solani AG-4 pathogens in Petri plates. Treatment with P. aureofaciens 63–28 alone increased the emergence rate, shoot fresh weight, shoot dry weight and root fresh weight at 7 days after inoculation, when compared to R. solani AG-4; P + R treatment showed similar effects. Peroxidase (POD) and β-1,3-glucanase activity of P. aureofaciens 63–28 treated roots increased by 41.1 and 49.9%, respectively, compared to control roots. POD was 26% greater in P + R treated roots than R. solani treated roots. Two POD isozymes (59 and 27 kDa) were strongly induced in P + R treated roots. The apparent molecular weight of chitinase from treated roots, as determined through SDS-PAGE separation and comparison with standards, was about 29 kDa. Five β-1,3-glucanase isozymes (80, 70, 50, 46 and 19 kDa) were observed in all treatments. These results suggest that inoculation of soybean plants with P. aureofaciens 63–28 elevates plant growth inhibition by R. solani AG-4 and activates PR-proteins, potentially through induction of systemic resistance mechanisms.

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Acknowledgements

This work was supported by an NSERC grant to Dr. Donald L Smith.

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Authors and Affiliations

  1. Department of Plant Science, McGill University, Macdonald Campus, 21,111 Lakeshore Road, St Anne-de-Bellevue, QC, Canada, H9X 3V9

    Woo Jin Jung, Fazli Mabood & Donald L. Smith

  2. Department of Animal Science, Institute of Agricultural Science & Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju, 500-757, Korea

    Tae Hwan Kim

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  1. Woo Jin Jung
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  2. Fazli Mabood
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  3. Tae Hwan Kim
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  4. Donald L. Smith
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Correspondence to Donald L. Smith.

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Jung, W.J., Mabood, F., Kim, T.H. et al. Induction of pathogenesis-related proteins during biocontrol of Rhizoctonia solani with Pseudomonas aureofaciens in soybean (Glycine max L. Merr.) plants. BioControl 52, 895–904 (2007). https://doi.org/10.1007/s10526-007-9089-x

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