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Effect of restricted feeding on nocturnality and daily leptin rhythms in OVLT in aged male Wistar rats

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Abstract

Circadian system has direct relevance to the problems of modern lifestyle, shift workers, jet lag etc. To understand non-photic regulation of biological clock, the effects of restricted feeding (RF) on locomotor activity and daily leptin immunoreactivity (ir) rhythms in three age groups [3, 12 and 24 months (m)] of male Wistar rats maintained in light:dark (LD) 12:12 h conditions were studied. Leptin-ir was examined in the suprachiasmatic nucleus (SCN), the medial preoptic area (MPOA) and organum vasculosum of the lamina terminalis (OVLT). Reversal of feeding time due to restricted food availability during daytime resulted in switching of the animals from nocturnality to diurnality with significant increase in day time activity and decrease in night time activity. The RF resulted in % diurnality of approximately 32, 29 and 73 from % nocturnality of 82, 92 and 89 in control rats of 3, 12 and 24 m age, respectively. The increase in such switching from nocturnality to diurnality with restricted feeding was found to be robust in 24 m rats. The OVLT region showed daily leptin-ir rhythms with leptin-ir maximum at ZT-0 in all the three age groups. However leptin-ir levels were minimum at ZT-12 in 3 and 12 m though at ZT-18 in 24 m. In addition the mean leptin-ir levels decreased with increase in food intake and body weight significantly in RF aged rats. Thus we report here differential effects of food entrained regulation in switching nocturnality to diurnality and daily leptin-ir rhythms in OVLT in aged rats.

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Acknowledgements

The work was supported by ICMR (Ref. No. BMS/NTF/14/2006-2007, DST (Do No. SR/SO/AS-47/2004), UGC (ref: F.No. 32-613/2006/(SR) and UPE grants to AJ. VDK Reddy is thankful to ICMR for fellowship. Authors are thankful to Prof. W. J. Schwartz for critical reading and valuable suggestions during preparation of this manuscript.

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  1. Neurobiology and Molecular Chronobiology Laboratory, Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, Andhra Pradesh, India

    V. D. K. Reddy & Anita Jagota

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  1. V. D. K. Reddy
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Reddy, V.D.K., Jagota, A. Effect of restricted feeding on nocturnality and daily leptin rhythms in OVLT in aged male Wistar rats. Biogerontology 15, 245–256 (2014). https://doi.org/10.1007/s10522-014-9494-3

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