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Detection of an aging-related increase in advanced glycation end products in fast- and slow-twitch skeletal muscles in the rat

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Abstract

Glycation, a non-enzymatic addition of reducing sugars to ε-amino groups of proteins, is a post-translational modification that results in the formation of irreversible advanced glycation end products (AGEs). Ageing related decline in myofibrillar protein function is effected by a number of structural and functional modifications including glycation. Functional properties of skeletal muscles, such as maximum velocity of unloaded shortening, are known to be profoundly affected by ageing at the motor unit, cellular and tissue levels. However, the contribution of protein modifications to a decline in muscle function is not well understood. In this study we measured AGEs of intracellular and sarcolemmal proteins, using an anti-AGE antibody in soleus (SOL) and extensor digiotorum longus (EDL) muscles of male and female rats of five different age groups. Using a fluorescent secondary antibody to visualize AGEs in the confocal microscope, we found that myosin is glycated in both fiber types in all age groups; an ageing related increase in AGEs was observed in both intracellular and sarcolemmal regions in all age groups, with the exception of sarcolemma of SOL (unchanged) and EDL (reduced) in female rats; the greatest concentration of AGEs was found intracellularly in the SOL of the oldest age group (27–30) of females. While an ageing related decline in motor properties can be partially attributed to the observed increase in myofibrillar protein glycation, our results also indicate that intracellular and the less well studied sarcolemmal protein modification likely contribute to an aging-related decline in muscle function. Further studies are required to establish a link between the observed ageing related increase in glycation and muscle function at the motor unit, cellular and tissue levels.

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Acknowledgments

We are grateful to Dr. Bertrand Friguet for providing us with the anti-AGE-RNAse antibody. This study was supported by grants from the European Commission (MyoAge, EC Fp7 CT-223756 and COST CM1001), STINT, Swedish Research Council (8651), NIH (AR 045627, AR 047318, AG014731), and King Gustaf V and Queen Victoria’s Foundation to L.L.

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  1. Department of Biobehavioral Health, The Pennsylvania State University, University Park, PA, USA

    B. Ramamurthy & L. Larsson

  2. Department of Neuroscience, Clinical Neurophysiology, Uppsala University, 751 85, Uppsala, Sweden

    L. Larsson

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  1. B. Ramamurthy
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  2. L. Larsson
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Correspondence to L. Larsson.

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Ramamurthy, B., Larsson, L. Detection of an aging-related increase in advanced glycation end products in fast- and slow-twitch skeletal muscles in the rat. Biogerontology 14, 293–301 (2013). https://doi.org/10.1007/s10522-013-9430-y

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  • DOI: https://doi.org/10.1007/s10522-013-9430-y

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