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Cultured senescent myoblasts derived from human vastus lateralis exhibit normal mitochondrial ATP synthesis capacities with correlating concomitant ROS production while whole cell ATP production is decreased

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Abstract

The free radical theory of aging says that increased oxidative stress and mitochondrial dysfunction are associated with old age. In the present study we have investigated the effects of cellular senescence on muscle energetic by comparing mitochondrial content and function in cultured muscle satellite cells at early and late passage numbers. We show that cultured muscle satellite cells undergoing senescence express a reduced mitochondrial mass, decreased whole cell ATP level, normal to increased mitochondrial ATP production under ATP utilization, increased mitochondrial membrane potential and increased superoxide/mitochondrial mass and hydrogen peroxide/mitochondrial mass ratios. Moreover, the increased ROS production correlates with the corresponding mitochondrial ATP production. Thus, myotubes differentiated from human myoblasts undergoing senescence have a reduced mitochondrial content, but the existent mitochondria express normal to increased functional capabilities. The present data suggest that the origin of aging lies outside the mitochondria and that a malfunction in the cell might be preceding and initiating the increase of mitochondrial ATP synthesis and concomitant ROS production in the single mitochondrion in response to decreased mitochondrial mass and reduced extra-mitochondrial energy supply. This then can lead to the increased damage of DNA, lipids and proteins of the mitochondria as postulated by the free radical theory of aging.

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Acknowledgments

Lynfort I and Agergaard J provided excellent technical assistance. Højlund K and Levin K were thanked for muscle biopsies. The Danish Medical Research Council, the Novo Nordisk Foundation and the free research funds of Odense University Hospital were thanked for financial support.

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Authors and Affiliations

  1. Department of Pathology, Laboratory for Molecular Physiology and Department of Endocrinology, Odense University Hospital, 5000, Odense, Denmark

    Michael Gaster

  2. Department of Pathology, Laboratory for Molecular Physiology, Odense University Hospital, 5000, Odense, Denmark

    Ariane D. Minet

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  1. Ariane D. Minet
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  2. Michael Gaster
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Correspondence to Michael Gaster.

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Minet, A.D., Gaster, M. Cultured senescent myoblasts derived from human vastus lateralis exhibit normal mitochondrial ATP synthesis capacities with correlating concomitant ROS production while whole cell ATP production is decreased. Biogerontology 13, 277–285 (2012). https://doi.org/10.1007/s10522-012-9372-9

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  • DOI: https://doi.org/10.1007/s10522-012-9372-9

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