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Coordinate expression of the human pregnancy-specific glycoprotein gene family during induced and replicative senescence

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Abstract

Pregnancy-specific glycoproteins (PSGs) comprise a family of highly similar polypeptides encoded by 11 transcriptionally active genes that compactly cluster on band 19q13.2. All members of the PSG family were found to be markedly up-regulated by addition of 5-bromodeoxyuridine in HeLa cells. Similarly, all of the members were markedly up-regulated during replicative senescence in normal human fibroblasts. Promoter analysis of the PSG1, 4, and 11 genes in HeLa cells did not reveal a cis-regulatory element responsive to 5-bromodeoxyuridine in their 5′-flanking sequences. These results suggest that the PSG genes are regulated at a level of higher order chromatin structure besides by a signal of pregnancy.

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Abbreviations

PSG:

Pregnancy-specific glycoprotein

BrdU:

5-Bromodeoxyuridine

PDL:

Population doubling level

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Authors and Affiliations

  1. International Graduate School of Arts and Sciences, Yokohama City University, Seto 22-2, Kanazawa-ku, Yokohama, Kanagawa, 236-0027, Japan

    Morio Endoh, Yusuke Kobayashi, Yoshimi Yamakami, Ryuzo Yonekura, Michihiko Fujii & Dai Ayusawa

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  1. Morio Endoh
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  2. Yusuke Kobayashi
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  3. Yoshimi Yamakami
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  4. Ryuzo Yonekura
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  5. Michihiko Fujii
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  6. Dai Ayusawa
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Correspondence to Dai Ayusawa.

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Endoh, M., Kobayashi, Y., Yamakami, Y. et al. Coordinate expression of the human pregnancy-specific glycoprotein gene family during induced and replicative senescence. Biogerontology 10, 213–221 (2009). https://doi.org/10.1007/s10522-008-9173-3

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  • DOI: https://doi.org/10.1007/s10522-008-9173-3

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