Abstract
We isolated highly purified CD8+CD28+ and CD8+CD28− T cell populations from healthy young and elderly persons for gene expression profiling using Affymetrix oligonucleotide microarrays. We demonstrate that the gene expression profile of CD8+CD28− T cells is very similar in young and elderly persons. In contrast, CD8+CD28+ in elderly differ from CD8+CD28+ in young persons. Hierarchical clustering revealed that CD8+CD28+ in elderly are located between CD8+CD28+ in young and CD8+CD28− (young and old) T cells regarding their differentiation state. Our study demonstrates a dichotomy of gene expression levels between CD8+CD28+ T cells in young and elderly persons but a similarity between CD8+CD28− T cells in young and elderly persons. As CD8+CD28+ T cells from elderly and young persons are distinct due to a different composition of the population, these results suggest that the gene expression profile does not depend on chronological age but depends on the differentiation state of the individual cell types.
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Acknowledgements
The authors thank Michael Keller and Brigitte Jenewein for collecting blood samples. This work was supported by the Austrian Science Fund (Projects S9308-B05 and S9309-B05
).
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Lazuardi, L., Herndler-Brandstetter, D., Brunner, S. et al. Microarray analysis reveals similarity between CD8+CD28− T cells from young and elderly persons, but not of CD8+CD28+ T cells. Biogerontology 10, 191–202 (2009). https://doi.org/10.1007/s10522-008-9167-1
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DOI: https://doi.org/10.1007/s10522-008-9167-1