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Heme oxygenase-1 and interleukin-11 are overexpressed in stress-induced premature senescence of human WI-38 fibroblasts induced by tert-butylhydroperoxide and ethanol

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Abstract

Acute repeated exposures to subcytotoxic concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of human diploid fibroblasts. In the present work we found an increased mRNA and protein level of interleukin-11 and heme oxygenase-1 in premature senescence of WI-38 human diploid foetal lung fibroblasts induced by both tert-butylhydroperoxide and ethanol. We tested whether interleukin-11 and heme oxygenase-1 could protect against tert-butylhydroperoxide- or ethanol-induced premature senescence when stable overexpression was established using a retroviral vector-based transduction. No protective effect was found against the decrease of the proliferative potential, the increase of the proportion of senescence-associated ß-galactosidase positive cells and the increase of the mRNA levels of six senescence-associated genes.

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Abbreviations

BSA:

Bovine serum albumin

cDNA:

Complementary deoxyribonucleic acid

HDFs:

Human diploid fibroblasts

HO:

Heme oxygenase

IGF-1:

Insulin-like growth factor-1

IL:

Interleukin

S-A ß-gal:

Senescence-associated ß-galactosidase

SIPS:

Stress-induced premature senescence

t-BHP:

tert-Butylhydroperoxide

TGF-ß1:

Transforming growth factor-ß1

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Acknowledgments

O. Toussaint and F. Debacq-Chainiaux are respectively Research Associate and Postdoctoral Researcher of the FNRS, Belgium. We acknowledge the Région Wallonne/FSE First-Europe projects “Arrayage”, “CosmUV” and First-DEI project “Cosmet-X”. We acknowledge the Région Wallonne “Réseaux II, Senegene” project, and « Nanotoxico » project. We also thank the European Commission (Link-Age Project, LSHM-CT-2005–523866, Geha Project, LSHM-CT-2004–503270).

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  1. Unit of Research on Cellular Biology (URBC), Department of Biology, University of Namur (FUNDP), Rue de Bruxelles, 5000, Namur, Belgium

    Thierry Pascal, Florence Debacq-Chainiaux, Emmanuelle Boilan, Noëlle Ninane, Martine Raes & Olivier Toussaint

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  1. Thierry Pascal
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  2. Florence Debacq-Chainiaux
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Correspondence to Olivier Toussaint.

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Pascal, T., Debacq-Chainiaux, F., Boilan, E. et al. Heme oxygenase-1 and interleukin-11 are overexpressed in stress-induced premature senescence of human WI-38 fibroblasts induced by tert-butylhydroperoxide and ethanol. Biogerontology 8, 409–422 (2007). https://doi.org/10.1007/s10522-007-9084-8

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