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Age- and gender-related alterations of the number and clonogenic capacity of circulating CD34+ progenitor cells

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Abstract

The aim of this study was to evaluate the peripheral representation and the clonogenic capacity of CD34+ progenitor cells from 130 healthy subjects (80 females and 50 males) ranging in age from 16 to 100 years. We demonstrated that the absolute number of circulating CD34+ cells progressively and significantly decreased with advancing age, with a 2–fold reduction in subjects aged more than 80 years. The number of granulocyte-macrophagic (CFU-GM), erytroid (BFU-E), and mixed (CFU-GEMM) colonies which developed from the number of CD34+ purified cells per ml, progressively and significantly decreased with advancing age. The reduction of both CD34+ cell number and clonogenic capacity during aging was statistically significant in males but not in females. When evaluated on a per cell bases, a significant age-related decrease in the number of CFU-GM colonies was observed in female but not in male subjects. Our study demonstrates the influence of gender on age-related alterations of the number and clonogenic capacity of CD34+ cells in the peripheral blood. This evidence deserves particular consideration for the future planning of stem cell therapy in age-associated debilitating diseases.

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Abbreviations

CFU-GM :

colony-forming unit-granulocyte macrophagic

BFU-E :

burst-forming unit

CFU-GEMM :

colony-forming unit granulocyte-erythroid- macrophagic-megakaryocytic

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Authors and Affiliations

  1. Laboratory of Tumor Immunology, INRCA Gerontol. Res. Dept., Immunology Center, Via Birarelli 8, 60121, Ancona, Italy

    Raffaella Moresi, Silvia Tesei, Laura Costarelli, Claudio Viticchi, Rosalia Stecconi, Giovanni Bernardini & Mauro Provinciali

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  1. Raffaella Moresi
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  2. Silvia Tesei
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  3. Laura Costarelli
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  4. Claudio Viticchi
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  5. Rosalia Stecconi
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  6. Giovanni Bernardini
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  7. Mauro Provinciali
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Correspondence to Mauro Provinciali.

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Moresi, R., Tesei, S., Costarelli, L. et al. Age- and gender-related alterations of the number and clonogenic capacity of circulating CD34+ progenitor cells. Biogerontology 6, 185–192 (2005). https://doi.org/10.1007/s10522-005-7954-5

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  • DOI: https://doi.org/10.1007/s10522-005-7954-5

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