The present paper evaluates the inclusion of a standard strain or outbred stock in multi-strain behavioral phenotyping protocols to perform the same role as the external standard in biochemical assay procedures. As potential standards, the F344 inbred strain and an outbred stock of Long Evans were tested with three other inbred strains. To evaluate the influence of rearing conditions on phenotype stability, one group of F344s was born at the University of Tsukuba, another, bred elsewhere and delivered to Tsukuba at 4 weeks of age. All animals were tested in open-field (OF), runway emergence (RE) and digging tests as adults. The results showed no influence of breeding or transportation history on OF and RE behavior of the two F344 groups, while there was evidence that digging behavior may be affected by the different rearing experience. The inclusion of a ‘standard strain or stock’ in phenotyping protocols involving multiple inbred strains or lines of rats, mice and flies has obvious advantages by providing a reference point for inter-laboratory comparisons. The properties of inbred strains and outbred stocks favorable to their use as standards are discussed.
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References
J. K. Belknap J. Riggan S. J. Cross E. R. Young E. J. Gallaher J. C. Crabbe (1993) ArticleTitleGenetic determinants of morphine activity and thermal responses in 15 inbred mouse strains Pharmacol. Biochem. Behav. 59 353–360
D. A. Blizard D. W. Bailey (1979) ArticleTitleGenetic correlation between open-field activity and defecation: analysis with CXB recombinant-inbred strains Behav. Genet. 9 349–357
D. A. Blizard C. T. Randt (1974) ArticleTitleGenotype interaction with undernutrition and external environment in early life Nature 251 705–707
D. A. Blizard D. J. Vandenbergh A. L. Jefferson C. D. Chatlos G. P. Vogler G. E. McClearn (2004) ArticleTitleEffects of periadolescent ethanol exposure on alcohol preference in two BALB substrains Alcohol 34 1–9
G.A Churchill D. C. Airey H. Allayee J. M. Angel A. D. Attie J. Beatty W. D. Beavis J. K. Belknap B. Bennett W. Berrettini A. Bleich M. Bogue K. W. Broman K. J. Buck E. Buckler M. Burmeister E. J. Chesler J. M. Cheverud S. Clapcote M.N Cook R. D. Cox J. C. Crabbe W. E. Crusio A. Darvasi C. F. Deschepper R. W. Doerge C. R. Farber J. Forejt D. Gaile S. J. Garlow H. Geiger H. Gershenfeld T. Gordon J. Gu W. Gu G. Haan Particlede N. L. Hayes C. Heller H. Himmelbauer R. Hitzemann K. Hunter H. C. Hsu F. A. Iraqi B. Ivandic H. J. Jacob R. C. Jansen K. J. Jepsen D. K. Johnson T. E. Johnson G. Kempermann C. Kendziorski M. Kotb R. F. Kooy B. Llamas F. Lammert J. M. Lassalle P. R. Lowenstein L. Lu A. Lusis K. F. Manly R. Marcucio D. Matthews J. F. Medrano D. R. Miller G. Mittleman B. A. Mock J. S. Mogil X. Montagutelli G. Morahan D. G. Morris R. Mott J. H. Nadeau H. Nagase R. S. Nowakowski B. F. O’Hara A. V. Osadchuk G. P. Page B. Paigen K. Paigen A. A. Palmer H. J. Pan L. Peltonen-Palotie J. Peirce D. Pomp M. Pravenec D. R. Prows Z. Qi R. H. Reeves J. Roder G. D. Rosen E. E. Schadt L. C. Schalkwyk Z. Seltzer K. Shimomura S. Shou M. J. Sillanpaa L. D. Siracusa H. W. Snoeck J. L. Spearow K. Svenson L. M. Tarantino D. Threadgill L. A. Toth W. Valdar F. P. Villena Particlede C. Warden S. Whatley R. W. Williams T. Wiltshire N. Yi D. Zhang M. Zhang F. Zou InstitutionalAuthorNameComplex Trait Consortium (2004) ArticleTitleThe Collaborative Cross, a community resource for the genetic analysis of complex traits Nat. Genet. 36 1133–1137
J. C. Crabbe E. J. Gallaher T. J. Phillips J. K. Belknap (1994) ArticleTitleGenetic determinants of sensitivity to ethanol in inbred mice Behav. Neurosci. 108 186–195
J. C. Crabbe E. J. Gallaher S. J. Cross J. K. Belknap (1998) ArticleTitleGenetic determinants of sensitivity to diazepam in inbred mice Behav. Neurosci. 112 668–677
J. C. Crabbe P. Metten E. J. Gallaher J. K. Belknap (2002) ArticleTitleGenetic determinants of sensitivity to pentobarbital in inbred mice Psychopharmacology 161 408–416
J. C. Crabbe D. Wahlsten B. C. Dudek (1999) ArticleTitleGenetics of mouse behavior: interactions with laboratory environment Science 284 1670–1672
J. C. DeFries J. P. Hegmann M. W. Weir (1966) ArticleTitleOpen-field behavior in mice: evidence for a major gene effect mediated by the visual system Science 154 1577–1579
W. N. Frankel L. Taylor B. Beyer B. L. Tempel H. S. White (2001) ArticleTitleElectroconvulsive thresholds of inbred mouse strains Genomics 74 306–312
O. Fujita Y. Annen A. Kitaoka (1994) ArticleTitleTsukuba High- and Low-Emotional strains of rats (Rattus norvegicus): an overview Behav. Genet. 24 389–415
S. C. Grubb G. A. Churchill M. A. Bogue (2004) ArticleTitleA collaborative database of inbred mouse strain characteristics Bioinformatics 20 2857–2859
N. D. Henderson (1967) ArticleTitlePrior treatment effects on open field behavior of mice – a genetic analysis Anim. Behav. 15 364–376
N. D. Henderson (1975) ArticleTitleShort exposures to enriched environments can increase genetic variability of behavior in mice Dev. Psychobiol. 9 549–553
N. D. Henderson (1980) Effects of early experience upon the behavior of animals: the second twenty-five years of research E. C. Simmel (Eds) Early experiences and early behavior: Implications for social development Academic Press New York 39–77
G. E. McClearn (1997) ArticleTitleHeterogeneous reference populations in animal model research in aging ILAR J. 38 119–123
G. E. McClearn J. R. Wilson W. Meredith (1970) The use of isogenic and heterogenic mouse stocks in behavioral research G. Lindzey D. D. Thiessen (Eds) Contributions to behavior-genetic analysis: The mouse as a prototype Appleton-Century-Crofts New York 3–22
L. A. Rodriguez R. Plomin D. A. Blizard B. C. Jones G. E. McClearn (1995) ArticleTitleAlcohol acceptance, preference, and sensitivity in mice: II. Quantitative trait loci mapping analysis using BXD recombinant inbred strains Alcohol. Clin. Exp. Res. 19 367–373
H. Shisa L. Lu H. Katoh A. Kawarai J. Tanuma Y. Matsushima H. Hiaia (1997) ArticleTitleThe LEXF: a new set of rat recombinant inbred strains between LE/Stm and F344 Mamm. Genome 8 324–327
R. W. Thompson (1953) ArticleTitleThe inheritance of behavioral differences in fifteen mouse strains Can. J. Psychol. 7 145–155
Wahlsten, D. W., and 19 co-authors (2003). Different data from different labs: lessons from studies of gene-environment interaction. J. Neurobiol. 54:283–311.
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Blizard, D.A., Wada, Y., Onuki, Y. et al. Use of A Standard Strain for External Calibration in Behavioral Phenotyping. Behav Genet 35, 323–332 (2005). https://doi.org/10.1007/s10519-005-3224-1
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DOI: https://doi.org/10.1007/s10519-005-3224-1