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Long-Term Persistence of Increased Number of γH2AX+ Peripheral Blood Lymphocytes in Monkeys Exposed to Negative Factors of Space Flights: Ionizing Radiation and Simulated Hypogravity

  • PRIMATOLOGY
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Bulletin of Experimental Biology and Medicine Aims and scope

We studied the influence of ionizing radiation and hypogravity as negative factors of space flights on DNA damage in peripheral blood lymphocytes of rhesus monkeys at different times after exposure (from 1 to 446 days). The proportion of cells with high numbers of DNA double-strand breaks (DSB), positive for the surrogate DSB marker-protein γH2AX, was monitored using flow cytometry. Some animals were exposed to 7-day antiorthostatic hypokinesia simulating hypogravity, the others to a combined effect of antiorthostatic hypokinesia, whole-body γ-irradiation (2.34 cGy/h, dose 1 Gy), and irradiation of the head with 12C ions (450 MeV, dose 1 Gy). Exposure to antiorthostatic hypokinesia led to a significant increase in the proportion of γH2AX+ lymphocytes only on the first day after exposure, whereas after combined exposure, increased numbers of damaged lymphocytes were recorded up to 42 days after exposure.

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Authors and Affiliations

  1. A. I. Burnasyan Federal Medical Biophysical Center, Federal Medical-Biological Agency of Russia, Moscow, Russia

    T. M. Blokhinа, E. I. Yashkina & A. N. Osipov

  2. N. N. Semenov Federal Research Center of Chemical Physics, Russian Academy of Sciences, Moscow, Russia

    T. M. Blokhinа & A. N. Osipov

  3. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia

    A. G. Belyaeva, A. A. Perevezentsev & A. S. Shtemberg

Authors
  1. T. M. Blokhinа
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  2. E. I. Yashkina
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  3. A. G. Belyaeva
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  4. A. A. Perevezentsev
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  5. A. S. Shtemberg
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  6. A. N. Osipov
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Correspondence to A. N. Osipov.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 172, No. 7, pp. 98-101, July, 2021

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Blokhinа, T.M., Yashkina, E.I., Belyaeva, A.G. et al. Long-Term Persistence of Increased Number of γH2AX+ Peripheral Blood Lymphocytes in Monkeys Exposed to Negative Factors of Space Flights: Ionizing Radiation and Simulated Hypogravity. Bull Exp Biol Med 172, 81–84 (2021). https://doi.org/10.1007/s10517-021-05336-8

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  • DOI: https://doi.org/10.1007/s10517-021-05336-8

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