The study examined transcriptional regulation of adrenal zona reticularis development in male Wistar rats exposed to low doses of endocrine disruptor dichlorodiphenyltrichloroethane (DDT) prenatally and postnatally. At pubertal age, zona reticularis demonstrated a retarded growth with a low proliferative activity of its cells. At this age, zona reticularis was characterized with overexpression of β-catenin by steroid-producing cells; a high percentage of cells with membrane and cytoplasmic localization of β-catenin, and reduced number of cells with nuclear β-catenin attesting to insufficient activation of Wnt signaling. Expression of transcriptional factor Oct4 by reticularis cells was down-regulated indicating their diminished proliferative potency. No significant alterations in Sonic Hedgehog expression were observed. Thus, DDT-provoked disorders of transcriptional regulation result in abnormal development of zona reticularis thereby disturbing sexual maturation in males.
Similar content being viewed by others
References
Technical Regulation of Customs Union TRCU 021/2011 On Food Safety. St. Peterburg, 2015. Russian.
Yaglova NV, Obernikhin SS, Nazimova SV, Yaglov VV. Role of Transcription Factor Oct4 in Postnatal Development and Function of the Adrenal Cortex. Bull. Exp. Biol. Med. 2019;167(4):568-573. doi: https://doi.org/10.1007/s10517-019-04573-2
Yaglova NV, Tsomartova DA, Obernikhin SS, Nazimova SV. The role of the canonical Wnt-signaling pathway in morphogenesis and regeneration of the adrenal cortex in rats exposed to the endocrine disruptor dichlorodiphenyltrichloroethane during prenatal and postnatal development. Biol. Bull. 2019;46(1):74-81.
Yaglova NV, Tsomartova DA, Yaglov VV. Differences in adrenal steroid hormones production in pubertal rats exposed to low doses of endocrine disruptor DDT during prenatal and postnatal development. Biomed. Khimiya. 2017;63(4):306-311. Russian.
Berthon A, Martinez A, Bertherat J, Val P. Wnt/β-catenin signalling in adrenal physiology and tumour development. Mol. Cell. Endocrinol. 2012;351(1):87-95.
Ching S, Vilain E. Targeted disruption of Sonic hedgehog in the mouse adrenal leads to adrenocortical hypoplasia. Genesis. 2009;47(9):628-637.
El-Hefnawy T, Hernandez C, Stabile LP. The endocrine disrupting alkylphenols and 4,4-DDT interfere with estrogen conversion and clearance by mouse liver cytosol. Reprod. Biol. 2017;17(3):185-192.
Finco I, Lerario AM, Hammer GD. Sonic hedgehog and WNT signaling promote adrenal gland regeneration in male mice. Endocrinology. 2018;159(2):579-596.
Gore AC, Chappell VA, Fenton SE, Flaws JA, Nadal A, Prins GS, Toppari J, Zoeller RT. EDC-2: The Endocrine Society’s second scientific statement on endocrine disrupting chemicals. Endocr. Rev. 2015;36(6):E1-E150.
Jacobson-Dickman E, Lee MM. The influence of endocrine disruptors on pubertal timing. Curr. Opin. Endocrinol. Diabetes Obes. 2009;16(1):25-30.
McClatchey AI, Yap AS. Contact inhibition (of proliferation) redux. Curr. Opin. Cell Biol. 2012;24(5):685-694.
Özen S, Darcan Ş. Effects of environmental endocrine disruptors on pubertal development. J. Clin. Res. Pediatr. Endocrinol. 2011;3(1):1-6.
Ribatti D. A revisited concept: Contact inhibition of growth. From cell biology to malignancy. Exp. Cell Res. 2017; 359(1):17-19.
Yates R, Katugampola H, Cavlan D, Cogger K, Meimaridou E, Hughes C, Metherell L, Guasti L, King P .Adrenocortical development, maintenance, and disease. Curr. Top. Dev. Biol. 2013;106:239-312.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 11, pp. 651-655, November, 2020
Rights and permissions
About this article
Cite this article
Yaglova, N.V., Obernikhin, S.S., Yaglov, V.V. et al. Low-Dose Exposure to Endocrine Disruptor Dichlorodiphenyltrichloroethane (DDT) Affects Transcriptional Regulation of Adrenal Zona Reticularis in Male Rats. Bull Exp Biol Med 170, 682–685 (2021). https://doi.org/10.1007/s10517-021-05132-4
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-021-05132-4