We studied the effects of social isolation for 1, 2 or 3 weeks on behavioral reactions of male rats. As social isolation is a common model for inducing depressive-like state in rodents, classical tests for depressive-like behavioral features were conducted: Porsolt forced swimming test and tests for anhedonia and social interest. None of the experimental groups showed statistically significant disorders in comparison with the control group kept under standard conditions. Thus, social isolation for up to 3 weeks did not cause behavioral abnormalities in male rats. Single housing can be used in other models of induction of depressive-like states, but the use of this paradigm as an independent model for the development of depressive-like behavior requires longer period of social isolation.
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References
Morozova AY, Zubkov EA, Storozheva ZI, Kekelidze ZI, Chekhonin VP. Effect of ultrasonic irradiation on the development of symptoms of depression and anxiety in rats. Bull. Exp. Biol. Med. 2013;154(6):740-743.
Khonicheva NM, Czabak-Garbacz R, Krupina NA. Behavioral consequences of isolation in early ontogeny in rats: selectivity of anxiety conditions. Zh. Vyssh. Nervn. Deyat. 2002;52(6):743-749. Russian.
Shabanov PD, Lebedev AA, Rusanovskii VV. Behavioral and Neurochemical Consequences of Social Isolation. Obzory po Klin. Neirofiziol. Lek. Ter. 2003;2(4):26-44. Russian.
Castagné V, Moser P, Porsolt RD, Chapter G. Behavioral assessment of antidepressant activity in rodents. Methods of Behavior Analysis in Neuroscience. Buccafusco JJ, ed. Boca Raton, 2009.
Djordjevic J, Djordjevic A, Adzic M, Radojcic MB. Effects of chronic social isolation on Wistar rat behavior and brain plasticity markers. Neuropsychobiology. 2012;66(2):112-119.
Fischer CW, Liebenberg N, Elfving B, Lund S, Wegener G. Isolation-induced behavioural changes in a genetic animal model of depression. Behav. Brain Res. 2012;230(1):85-91.
Lukkes JL, Watt MJ, Lowry CA, Forster GL. Consequences of post-weaning social isolation on anxiety behavior and related neural circuits in rodents. Front. Behav. Neurosci. 2009;3:18. doi: https://doi.org/10.3389/neuro.08.018.2009.
Moreau JL. Simulating the anhedonia symptom of depression in animals. Dialogues Clin. Neurosci. 2002;4(4):351-360.
Overstreet DH. Modeling depression in animal models. Methods Mol. Biol. 2012;829:125-144.
Reich CG, Taylor ME, McCarthy MM. Differential effects of chronic unpredictable stress on hippocampal CB1 receptors in male and female rats. Behav. Brain Res. 2009;203(2):264-269.
Slattery DA, Cryan JF. Using the rat forced swim test to assess antidepressant-like activity in rodents. Nat. Protoc. 2012;7(6):1009-1014.
Stepanichev M, Dygalo NN, Grigoryan G, Shishkina GT, Gulyaeva N. Rodent models of depression: neurotrophic and neuroinflammatory biomarkers. Biomed. Res. Int. 2014;2014. ID 932757. doi: https://doi.org/10.1155/2014/932757.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 165, No. 2, pp. 140-142, February, 2018
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Gorlova, A.V., Pavlov, D.A., Zubkov, E.A. et al. Three-Week Isolation Does Not Lead to Depressive-Like Disorders in Rats. Bull Exp Biol Med 165, 181–183 (2018). https://doi.org/10.1007/s10517-018-4125-7
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DOI: https://doi.org/10.1007/s10517-018-4125-7