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Expression of Bcl-2 Family Proteins in the Ovarian Follicular Apparatus in the Acute Period after Experimental Hyperthermia

  • MORPHOLOGY AND PATHOMORPHOLOGY
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Bulletin of Experimental Biology and Medicine Aims and scope

The expression of apoptosis regulators (proapoptotic protein Bad and anti-apoptotic protein Bcl-2) was analyzed and Bcl-2/Bad ratio in the follicular apparatus of the rat ovary was determined on day 3 after hyperthermia (rectal temperature 43.5°C). Hyperthermia in the catabolic phase leads to different degrees of activation of the molecular “switches” of apoptosis in cells of ovarian follicular epithelium. This was seen from increased intensity of immunohistochemical staining for Bad protein against the background of more pronounced expression of Bcl-2 protein. On day 3 after exposure to hyperthermia, Bcl-2/Bad ratio increased, which reflects antiapoptotic protection of cells and conditions for blockade of mitochondrial pathway of apoptosis in the follicular apparatus of the ovaries during the acute period after hyperthermia.

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References

  1. Michurina SV, Arkhipov SA, Kolesnikov SI. Hepatocyte apoptosis in rats exposed to benzo(a)pyrene. Bull. Exp. Biol. Med. 2014;158(1):150-152.

    Article  CAS  PubMed  Google Scholar 

  2. Michurina SV, Bochkareva AL, Belkin AD, Zhdanov AP, Bochkarev IG. Morphometric analysis of the vascular bed and yellow bodies in the ovaries at different times after the general controlled hyperthermia (experimental study). Khirurg. 2008;(6):9-13. Russian.

  3. Efremov AV, Pakhomova JuV, Pakhomov EA, Ibragimov RSh, Shorina GN. Patent RU No. 2165105. Method for carrying out experimental general hyperthermy modeling in small laboratory animals. Bull. No. 10. Published April 10, 2001.

  4. Aroyo A, Yavin S, Arav A, Roth Z. Maternal hyperthermia disrupts developmental competence of follicle-enclosed oocytes: in vivo and ex vivo studies in mice. Theriogenology. 2007;67(5):1013-1021.

    Article  CAS  PubMed  Google Scholar 

  5. Begriche K, Massart J, Robin MA, Borgne-Sanchez A, Fromenty B. Drug-induced toxicity on mitochondria and lipid metabolism: mechanistic diversity and deleterious consequences for the liver. J. Hepatol. 2011;54(4):773-794.

    Article  CAS  PubMed  Google Scholar 

  6. Belzacq AS, Vieira HL, Verrier F, Vandecasteele G, Cohen I, Prévost MC, Larquet E, Pariselli F, Petit PX, Kahn A, Rizzuto R, Brenner C, Kroemer G. Bcl-2 and Bax modulate adenine nucleotide translocase activity. Cancer Res. 2003;63(2):541-546.

    CAS  PubMed  Google Scholar 

  7. Bridges PJ, Brusie MA, Fortune JE. Elevated temperature (heat stress) in vitro reduces androstenedione and estradiol and increases progesterone secretion by follicular cells from bovine dominant follicles. Domest. Anim. Endocrinol. 2005;29(3):508-522.

    Article  CAS  PubMed  Google Scholar 

  8. Dewanjee S, Dua TK, Khanra R, Das S, Barma S, Joardar S, Bhattacharjee N, Zia-Ul-Haq M, Jaafar HZ. Water Spinach, Ipomoea aquatic (Convolvulaceae), Ameliorates Lead Toxicity by Inhibiting Oxidative Stress and Apoptosis. PLoS One. 2015;10(10):e0139831. doi: https://doi.org/10.1371/journal.pone.0139831.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Roth Z, Aroyo A, Yavin S, Arav A. The antioxidant epigallocatechin gallate (EGCG) moderates the deleterious effects of maternal hyperthermia on follicle-enclosed oocytes in mice. Theriogenology. 2008;70(6):887-897.

    Article  CAS  PubMed  Google Scholar 

  10. Stankiewicz AR, Lachapelle G, Foo CP, Radicioni SM, Mosser DD. Hsp70 inhibits heat-induced apoptosis upstream of mitochondria by preventing Bax translocation. J. Biol. Chem. 2005;280(46):38 729-38 739.

  11. Sun D, Li S, Wu H, Zhang M, Zhang X, Wei L, Qin X, Gao E Oncostatin M (OSM) protects against cardiac ischaemia/reperfusion injury in diabetic mice by regulating apoptosis, mitochondrial biogenesis and insulin sensitivity. J. Cell. Mol. Med. 2015;19(6):1296-1307.

  12. Tseng JK, Tang PC, Ju JC. In vitro thermal stress induces apoptosis and reduces development of porcine parthenotes. Theriogenology. 2006;66(5):1073-1082.

  13. Uchida S, Hotta H, Hanada T, Okuno Y, Aikawa Y. Effects of thermal stimulation, applied to the hindpaw via a hot water bath, upon ovarian blood flow in anesthetized nonpregnant rats. J. Physiol. Sci. 2007;57(4):227-233.

    Article  PubMed  Google Scholar 

  14. Wang Y, Chen Y, Zhang X, Cai G, An S, Wang X, Teng L, Wang D. Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway. Biomed. Res. Int. 2016;2016:9014364. doi: https://doi.org/10.1155/2016/9014364.

    PubMed  PubMed Central  Google Scholar 

  15. Yuan Y, Hao ZD, Liu J, Wu Y, Yang L, Liu GS, Tian JH, Zhu SE, Zeng SM. Heat shock at the germinal vesicle breakdown stage induces apoptosis in surrounding cumulus cells and reduces maturation rates of porcine oocytes in vitro. Theriogenology. 2008;70(2):168-178.

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Authors and Affiliations

  1. Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of the Federal Research Centre Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Novosibirsk, Russia

    S. V. Michurina, A. L. Bochkareva, S. A. Arkhipov & I. Yu. Ishchenko

  2. Research Center for Family Health and Human Reproduction Problems, Irkutsk, Russia

    S. I. Kolesnikov

Authors
  1. S. V. Michurina
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  2. S. I. Kolesnikov
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  3. A. L. Bochkareva
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  4. S. A. Arkhipov
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  5. I. Yu. Ishchenko
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Corresponding author

Correspondence to S. V. Michurina.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 164, No. 12, pp. 754-758, December, 2017

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Michurina, S.V., Kolesnikov, S.I., Bochkareva, A.L. et al. Expression of Bcl-2 Family Proteins in the Ovarian Follicular Apparatus in the Acute Period after Experimental Hyperthermia. Bull Exp Biol Med 164, 780–783 (2018). https://doi.org/10.1007/s10517-018-4079-9

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  • DOI: https://doi.org/10.1007/s10517-018-4079-9

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