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Combined Effect of Low-Intensity Helium-Neon Laser and X-Ray Radiation on in Vivo Cellular Response of the Whole Blood and Lymphoid Organs in Mice

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Bulletin of Experimental Biology and Medicine Aims and scope

We studied the effect of exposure to helium-neon laser (dose range 0.16-50 mJ/cm2) on activation of natural protection reserve in mice using the adaptive response test. DNA comets method revealed a protective response manifested in DNA damage level in whole blood leukocytes of mice and in lymphoid organs by the thymus and spleen weight index; preexposure to laser did not induce the adaptive response. ROS level in the whole blood was assessed by the level of zymosan-induced luminol chemiluminescence. In mice subjected to adaptive laser irradiation in doses of 0.16-5 mJ/cm2 followed by X-ray irradiation in a dose of 1.5 Gy, the activation index calculated as the ratio of induced to spontaneous area of luminescence was by 1.4 times lower than that in non-irradiated animals, which attested to reduced ROSgeneration reserve capacity of neutrophils.

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia

    S. I. Zaichkina, A. R. Dyukina, O. M. Rozanova, S. P. Romanchenko, N. P. Sirota, E. A. Kuznetsova, N. B. Simonova, S. S. Sorokina & D. T. Zakrzhevskaya

  2. Institute on Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia

    V. I. Yusupov & V. N. Bagratishvili

Authors
  1. S. I. Zaichkina
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  2. A. R. Dyukina
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  3. O. M. Rozanova
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  4. S. P. Romanchenko
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  5. N. P. Sirota
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  6. E. A. Kuznetsova
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  7. N. B. Simonova
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  8. S. S. Sorokina
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  9. D. T. Zakrzhevskaya
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  10. V. I. Yusupov
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  11. V. N. Bagratishvili
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Corresponding author

Correspondence to S. I. Zaichkina.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 161, No. 5, pp. 621-624, May, 2016

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Zaichkina, S.I., Dyukina, A.R., Rozanova, O.M. et al. Combined Effect of Low-Intensity Helium-Neon Laser and X-Ray Radiation on in Vivo Cellular Response of the Whole Blood and Lymphoid Organs in Mice. Bull Exp Biol Med 161, 679–682 (2016). https://doi.org/10.1007/s10517-016-3484-1

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  • DOI: https://doi.org/10.1007/s10517-016-3484-1

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