We studied the effects of DMSO and fibroblasts during HepaRG cell spheroid formation and conditions of their subsequent culturing on the levels of mRNA of the major cytochromes P450. A protocol of spheroid formation from differentiated HepaRG cells and their culturing in serum- and DMSO-free medium is developed.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 161, No. 1, pp. 138–142, January, 2016
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Aleksandrova, A.V., Burmistrova, O.A., Fomicheva, K.A. et al. Maintenance of High Cytochrome P450 Expression in HepaRG Cell Spheroids in DMSO-Free Medium. Bull Exp Biol Med 161, 120–124 (2016). https://doi.org/10.1007/s10517-016-3360-z
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DOI: https://doi.org/10.1007/s10517-016-3360-z