Skip to main content
SpringerLink
Log in

Creation of a Producent, Optimization of Expression, and Purification of Recombinant Yersinia Pseudotuberculosis L-Asparaginase

  • Immunology and Microbiology
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Recombinant E. coli strain producing Y. pseudotuberculosis Q66CJ2 (YpA) L-asparaginase II was created. Gene ansB homologue encoding Y. pseudotuberculosis IP 32953 L-asparaginase precursor was synthesized. The gene was cloned in pBad24 expression vector and expressed in E. coli BL21 (DE3) strain. Optimal conditions for the producer strain culturing were selected. An effective method for isolation and purifi cation of the enzyme by two-staged column chromatography was developed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. O. Yu. Abakumova, O. V. Podobed, A. A. Borisova, et al., Biomed. Khim., 54, No. 6, 712-719 (2008).

    PubMed  CAS  Google Scholar 

  2. Ju. A. Gladilina, N. N. Sokolov, and Yu. V. Krasotkina, Ibid., No. 4, 482-486 (2008).

  3. M. V. Pokrovskaya, N. N. Sokolov, V. S. Pokrovsky, et al., A Method for Detection of L-Asparaginase Producer Bacterial Strains. Decision on Granting an Invention Patent of April 7, 2010, in response to application No. 2008122007.

  4. D. Cappelletty, L. R. Chiarelli, M. V. Pasquetto, et al., Biochem. Biophys. Res. Commun., 377, No. 4, 1222-1226 (2008).

    Article  Google Scholar 

  5. E. F. Hartree, Anal. Biochem., 192, 215-218 (1991).

    Article  Google Scholar 

  6. J. Krasotkina, A. A. Borisova, Y. V. Gervaziev, and N. N. Sokolov, Biotechnol. Appl. Biochem., 39, Pt. 2, 215-221 (2004).

    Article  PubMed  CAS  Google Scholar 

  7. A. A. Pritsa, K. T. Papazisis, A. H. Kortsaris, et al., Anticancer Drugs, No. 12, 137-142 (2001).

  8. R. B. Reinert, L. M. Oberle, S. A. Wek, et al., J. Biol. Chem., 281, No. 42, 31,222-31,233 (2006).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Genetics, Moscow, Russia

    K. V. Sidoruk & V. G. Bogush

  2. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia

    V. S. Pokrovsky, A. A. Borisova, N. M. Omeljanuk, S. S. Aleksandrova, M. V. Pokrovskaya, Ju. A. Gladilina & N. N. Sokolov

Authors
  1. K. V. Sidoruk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. S. Pokrovsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. A. Borisova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. M. Omeljanuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. S. Aleksandrova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. V. Pokrovskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ju. A. Gladilina
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. V. G. Bogush
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. N. N. Sokolov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. S. Pokrovsky.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 152, No. 8, pp. 179-183, August, 2011

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sidoruk, K.V., Pokrovsky, V.S., Borisova, A.A. et al. Creation of a Producent, Optimization of Expression, and Purification of Recombinant Yersinia Pseudotuberculosis L-Asparaginase. Bull Exp Biol Med 152, 219–223 (2011). https://doi.org/10.1007/s10517-011-1493-7

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-011-1493-7

Key Words

Search

Navigation