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Changes in the Profiles of Chemokines Secreted by Endothelial Cells and Monocytes under Different Coculturing Conditions

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Secretion of chemokines under different conditions of monocyte and endothelial cell coculturing was compared. Secretion of all the studied chemokines was recorded in cocultures: IL-8/CXCL-8, MCP-1/CCL2, RANTES/CCL5, and IP-10/CXCL10. The presence of TNF-α increased the concentrations of all chemokines, the concentrations of IL-8/CXCL-8, MCP-1/CCL2, and IP-10/CXCL10 decreased significantly in transendothelial migration. Addition of IFN-γ to cocultures significantly increased only IP-10/CXCL10 concentration; in transendothelial migration, the concentration P-10/CXCL10 decreased, while the concentrations of RANTES/CCL5 and MCP-1/CCL2 increased. Cell coculturing with IL-4 reduced the concentrations of all chemokines; the concentration of RANTES/CCL5 significantly increased in transendothelial migration. These results demonstrate the important role of monocyteendothelial interactions in the regulation of the constitutive and cytokine-induced secretion of chemokines.

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Authors and Affiliations

  1. Institute of Experimental Medicine, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia

    E. A. Starickova & I. S. Freidlin

  2. D. O. Ott Institute of Obstetrics and Gynecology, St. Petersburg, Russia

    D. I. Sokolov & S. A. Selkov

Authors
  1. E. A. Starickova
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  2. D. I. Sokolov
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  3. S. A. Selkov
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  4. I. S. Freidlin
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Corresponding author

Correspondence to E. A. Starickova.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 150, No. 10, pp. 420–423, October, 2010

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Starickova, E.A., Sokolov, D.I., Selkov, S.A. et al. Changes in the Profiles of Chemokines Secreted by Endothelial Cells and Monocytes under Different Coculturing Conditions. Bull Exp Biol Med 150, 446–449 (2011). https://doi.org/10.1007/s10517-011-1165-7

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  • DOI: https://doi.org/10.1007/s10517-011-1165-7

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