Abstract
Blood ammonia concentration increased in the portal vein (by 1.4 times) and inferior vena cava (caudal to the renal vein inflow, by 2.2 times; and cranial to the hepatic vein inflow, by 2.5 times) of rats 3 h after intragastric administration of 16.57 M ethanol solution (446 mmol/kg, ≈ 1.4 LD50/48 h). Ammonia concentration in mixed blood samples (post-decapitation) increased by 39%. The rate of ammonia accumulation was 3-fold higher in an intraperitoneal lavage solution. Three-hour exposure of ethanol-treated animals to atmospheric ammonia (0.84–1.07 mg/liter, i.e. ≈ 1/8LC50 for intact rats) was followed by a 2.4-fold increase in blood ammonia concentration as compared to specimens of the ethanol group. Ammonia inhalation potentiated the lethal effect of ethanol (dose variation factor 0.81), suppressed external respiration, and decreased oxygen consumption. Our results indicate that kinetic changes in endogenous ammonia have an adverse effect on the outcome of alcohol intoxication in rats.
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References
Hazardous Industrial Substances, Eds. N. V. Lazarev and I. D. Gadaskina [in Russian], Leningrad (1977), Vol. 3, 7th Edition, p. 89.
V. L. Rejniuk, T. V. Schafer, R. V. Ovsep'yan, and Ju. Ju. Ivnitsky, Byull. Eksp. Biol. Med., 143, No. 6, 634–637 (2007).
T. V. Schafer, Ju. Ju. Ivnitsky, and V. N. Malakhovsky, Ibid., 135, No. 4, 419–422 (2003).
N. K. Aagaard, T. Thogersen, T. Grofte, et al., Alcohol Clin. Exp. Res., 28, No. 5, 697–701 (2004).
K. Adachia, T. Matsuhashia, Yu. Nishizawaa, et al., Biochem. Pharmacol., 50, No. 9, 1391–1399 (1995).
Ju. Ju. Ivnitsky, V. L. Rejniuk, T. V. Schafer, and V. N. Malakhovsky, Toxicol., 224, 184–190 (2006).
V. Mohanachari, K. S. Reddy, and K. Indira, Toxicol. Lett., 20, No. 2, 225–228 (1984).
M. C. Vemuri and K. Indira, Biochem. Med. Metab. Biol., 36, No. 1, 8–13 (1986).
A. Watanabe and Y. Kuwabara, Res. Exp. Med. (Berl.), 194, No. 3, 157–164 (1994).
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Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 145, No. 6, pp. 688–690, June, 2008
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Rejniuk, V.L., Schafer, T.V. & Ivnitsky, J.J. Ammonia potentiates the lethal effect of ethanol on rats. Bull Exp Biol Med 145, 741–743 (2008). https://doi.org/10.1007/s10517-008-0191-6
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DOI: https://doi.org/10.1007/s10517-008-0191-6