Skip to main content
SpringerLink
Log in

Cultures of hESM human embryonic stem cells: Chromosomal aberrations and karyotype stability

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

Cytogenetic analysis of karyotypes of hESM01-hESM04 human embryonic stem cells and substrains derived from these strains showed that all these strains retained normal karyotype during long-term culturing. Two substrains of embryonic stem cells with chromosome aberrations indicating clonal origin of these strains were detected. The potentialities of using analysis of chromosome variability of embryonic stem cells for evaluation of predisposition of the corresponding genotypes to the formation of chromosome abnormalities are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M. A. Prokhorovich, A. G. Shilov, N. B. Rubtsov, et al., Cell Cultures. Information Bulletin [in Russian], St. Petersburg (2006), No. 21, pp. 65–68.

  2. J. Buzzard, N. Gough, and J. Crook, Nature Biotechnol., 22, No. 4, 381–382 (2004).

    Article  CAS  Google Scholar 

  3. J. Draper, K. Smith, P. Gokhale, et al., Ibid., 22, No. 1, 53–54 (2004).

    Article  CAS  Google Scholar 

  4. M. Imreh, K. Gertow, J. Cederval, et al., J. Cell. Biochem., 99, 508–516 (2006).

    Article  PubMed  CAS  Google Scholar 

  5. M. A. Lagarkova, P. Y. Volchkov, A. V. Lyakisheva, et al., Cell Cycle, 5, No. 4, 416–420 (2006).

    PubMed  CAS  Google Scholar 

  6. A. Maitra, D. Arking, N. Shivapurkar, et al., Nature Genet., 37, 1099–1103 (2005).

    Article  PubMed  CAS  Google Scholar 

  7. M. Mitalipova, R. Rao, D. Hoyer, et al., Nat. Biotechnol., 23, No. 1, 19–20 (2005).

    Article  PubMed  CAS  Google Scholar 

  8. P. Stankievicz, I. Brozek, Z. Helias-Rodzewicz, et al., Am. J. Med. Genetics, 101, 226–239 (2001).

    Article  Google Scholar 

  9. R. Verma and B. Arvind, Human Chromosomes. Principles and Techniques, New York (1994), pp. 72–172.

Download references

Author information

Authors and Affiliations

  1. Novosibirsk State University, Russia

    M. A. Prokhorovich & N. B. Rubtsov

  2. N. I. Vavilov Institute of General Genetics, Russian Academy of Medical Sciences, Russia

    M. A. Lagar’kova & S. L. Kiselyov

  3. Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Novosibirsk

    M. A. Prokhorovich, A. G. Shilov, T. V. Karamysheva & N. B. Rubtsov

Authors
  1. M. A. Prokhorovich
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Lagar’kova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. G. Shilov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. V. Karamysheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. L. Kiselyov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. N. B. Rubtsov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. B. Rubtsov.

Additional information

__________

Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 3, pp. 143–146, March, 2007

Rights and permissions

Reprints and permissions

About this article

Cite this article

Prokhorovich, M.A., Lagar’kova, M.A., Shilov, A.G. et al. Cultures of hESM human embryonic stem cells: Chromosomal aberrations and karyotype stability. Bull Exp Biol Med 144, 126–129 (2007). https://doi.org/10.1007/s10517-007-0271-z

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-007-0271-z

Key Words

Search

Navigation