Abstract
The effects of YB-1 gene on the expression level of P-glycoprotein and drug resistance of tumor cells were studied in cultured HCT116 colon cancer cells. Transitory transfection of chimeric YB-1/GFP gene rendered HCT116 cells a selective advantage in a medium with vinblastine, which caused translocation of the chimeric protein into cell nuclei. This was paralleled by an increase in the expression of P-glycoprotein (multiple drug resistance protein).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
A. A. Stavrovskaya, Biokhimiya, 65, 112–126 (2000).
R. C. Bargou, K. Jurchott, C. Wagener, et al., Nat. Med., 3, No. 4, 447–450 (1997).
H. A. Chansky, M. Hu, D. D. Hickstein, and L. Yang, Cancer Res., 61, No. 9, 3586–3590 (2001).
E. K. Davydova, V. M. Evdokimova, L. P. Ovchinnikov, and J. W. Hershey, Nucleic Acids Res., 25, No. 14, 2911–2916 (1997).
V. Evdokimova, P. Ruzanov, H. Imataka, et al., EMBO J., 20, No. 19, 5491–5502 (2001).
Z. Hu, S. Jin, and K. W. Scotto, J. Biol. Chem., 275, No. 4, 2979–2985 (2000).
K. Kohno, H. Izumi, T. Uchiumi, et al., Bioessays, 25, No. 7, 691–698 (2003).
Y. Oda, A. Sakamoto, N. Shinohara, et al., Clin. Cancer Res., 4, No. 9, 2273–2277 (1998).
T. Ohga, K. Koike, M. Ono, et al., Cancer Res., 56, No. 18, 4224–4228 (1996).
T. Ohga, T. Uchiumi, Y. Makino, et al., J. Biol. Chem., 273, No. 11, 5997–6000 (1998).
K. Shibahara, K. Sugio, T. Osaki, et al., Clin. Cancer Res., 7, No. 10, 3151–3155 (2001).
K. Shibao, H. Takano, Y. Nakayama, et al., Int. J. Cancer, 83, No. 6, 732–737 (1999).
M. A. Skabkin, O. I. Kiselyova, K. G. Chernov, et al., Nucleic Acids Res., 32, No. 18, 5621–5635 (2004).
U. Stein, S. Bergmann, G. L. Scheffer, et al., Oncogene, 24, No. 22, 3606–3618 (2005).
U. Stein, K. Jurchott, W. Walther, et al., J. Biol. Chem., 276, No. 30, 28,562–28,569 (2001).
Author information
Authors and Affiliations
Corresponding author
Additional information
__________
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 143, No. 4, pp. 442–445, April, 2007
Rights and permissions
About this article
Cite this article
Vaiman, A.V., Stromskaya, T.P., Rybalkina, E.Y. et al. Development of drug resistance in the population of colon cancer cells under the effect of multifunctional protein YB-1. Bull Exp Biol Med 143, 463–466 (2007). https://doi.org/10.1007/s10517-007-0157-0
Received:
Issue Date:
DOI: https://doi.org/10.1007/s10517-007-0157-0