Abstract
The present study investigated the therapeutic effects of turmeric on the health of rohu (Labeo rohita) challenged with pathogenic Aeromonas veronii. The fish were fed on diets supplemented with different levels of turmeric for 120 days and then challenged with pathogenic A. veronii for 2 weeks, during which fish continued to receive respective turmeric-supplemented diets. Following trial, serum immune parameters and histomorphology of tissues including the intestine, liver, kidney, and gills were examined. The study showed severe effect of A. veronii in TM0 (positive control, basal diet without turmeric, and bacteria injected) treatment compared to TMN (negative control, basal diet without turmeric, and no bacteria injected). The treatments from TM5-TM20 (turmeric-supplemented feed at 5–20-g/kg feed with bacterial injection) showed less intensity of disease symptoms and significantly increased immune response with the increase in turmeric supplementation compared to TM0. In addition, the disease symptoms at tissue histomorphology decreased as the turmeric supplementation increased. Highest supplementation of turmeric (20 g/kg feed) showed reduced bacterial pathogenicity by maintaining nearly normal micro architecture of tissues compared to other turmeric-supplemented groups. The study concludes that A. veronii can infect L. rohita and can cause mortality unless proper preventive measures are practiced. Therefore, supplementation of turmeric could be recommended in aquaculture systems through feed to prevent disease impact.
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Acknowledgments
The authors express their gratitude to the Dean, College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India, for providing the field and laboratory facilities to perform the research. Authors also thank two anonymous reviewers for providing constructive comments on the earlier draft manuscript, which has significantly improved manuscript quality and Dr. Jawahar G. Patil, University of Tasmania, Australia, for help improving the manuscript presentation.
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There is no fishspecific information in the guidelines of Committee for the Purpose of Control and Supervision of Experiments on Animals, Ministry of Environment, Forest and Climate Change, Government of India, and it is not mandatory to obtain ethical clearance for fish in India. However, authors followed all the applicable international, national, and/ or institutional guidelines for the care and use of animals..
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Kaur, A., Holeyappa, S.A., Bansal, N. et al. Ameliorative effect of turmeric supplementation in feed of Labeo rohita (Linn.) challenged with pathogenic Aeromonas veronii. Aquacult Int 28, 1169–1182 (2020). https://doi.org/10.1007/s10499-020-00518-z
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DOI: https://doi.org/10.1007/s10499-020-00518-z