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Use of agar-bound microparticulate diet as alternative food for tropical abalone, Haliotis asinina (Linnaeus 1758) post-larvae in large-scale cultures

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Abstract

The efficacy of using agar-bound microparticulate diet (A-MPD) as alternative food for abalone Haliotis asinina Linne post-larvae in large-scale culture was investigated. Larvae sourced from the hatchery-bred (HB) and wild-sourced (WS) broodstock were fed with either diatoms (TMT1-NF), agar-bound microparticulate diet (TMT2-A-MPD), or a combination of both feeds (TMT3-NF + A-MPD) in six 2-m3 tanks replicated over time. Three hundred thousand veliger larvae were stocked/tank containing 80 corrugated plates with mucus trails hanging on bamboo poles. Feeds were given at 0900 h starting at day 3 with seawater flow through introduced every 1400 h starting day 5. Two-way analysis of variance determined significant differences (p < 0.05) in survival and shell length between larval sources and feed types. Tukey’s post hoc test established differences among treatment means. At day 30, survival for HB- and WS-sourced larvae was significantly higher (42%) in TMT3 compared with TMT2 having 35% for HB and 38% for WS (p < 0.05). Larvae fed with TMT1 had significantly lowest survival among the three treatments. Survival at 60 and 90 days did not show significant difference for TMT2 and TMT3 regardless of broodstock source. Post-larval shell growth (90 days), from both sources fed with TMT2 and TMT3, was significantly higher than TMT1 (p < 0.05). Larval performance did not show any significant interactions between HB and WS broodstock. The use of A-MPD alone or in combination may elicit improvement in survival and shell length growth in abalone larvae regardless of larval sources. A-MPD may be used as full or partial replacements to diatoms as alternative food for abalone post-larvae in large-scale culture.

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Abbreviations

HB:

Hatchery bred

WS:

Wild source

TMT1-NF:

Treatment 1-natural food (1:1 Cocconeis/Nitzschia)

TMT2-A-MPD:

Treatment 2-agar-bound microparticulate diet

TMT3-NF + A-MPD:

Treatment 3-natural food + agar-bound microparticulate diet

CCA:

Crustose coralline algae

FeCl3 :

Ferric chloride

Na2 EDTA:

Sodium ethylenediaminetetraacetic acid

SEAFDEC/AQD:

Southeast Asian Fisheries Development Center/Aquaculture Department

DOST:

Department of Science and Technology

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Acknowledgements

The authors would like to thank SEAFDEC/AQD (study code 5309-TTV-MO-312T) through its chief Dr. Felix Ayson and DOST for the funds provided; the SEAFDEC/AQD Abalone hatchery staff: Mr. Rafael Barrido, Mr. Ramil Piloton, and Mr. Nestor Bayona for their valuable suggestions in carrying out post-larval rearing work; Ms. Rena Santizo and Ms. Analyn Asutilla for their significant help and contribution in the study; and Mr. Richard Tantiado for the technical assistance in the conduct of experiment.

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Authors and Affiliations

  1. Aquaculture Department, Southeast Asian Fisheries Development Center, Tigbauan, Iloilo, Philippines

    MN Bautista-Teruel, JRH Maquirang & MR dela Peña

  2. College of Arts and Sciences, University of the Philippines in the Visayas, Miag-ao, Iloilo, Philippines

    VT Balinas

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  1. MN Bautista-Teruel
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  3. MR dela Peña
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Correspondence to MN Bautista-Teruel.

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Bautista-Teruel, M., Maquirang, J., dela Peña, M. et al. Use of agar-bound microparticulate diet as alternative food for tropical abalone, Haliotis asinina (Linnaeus 1758) post-larvae in large-scale cultures. Aquacult Int 25, 1239–1252 (2017). https://doi.org/10.1007/s10499-017-0110-9

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