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1H-NMR metabolite profiling of abalone digestive gland in response to short-term starvation

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Abstract

The effect of a short-term feeding and starvation experiment on juvenile abalone (Haliotis rubra × H. laevigata) was investigated (average length = 67 mm; average weight = 48 g). All aquaculture experiments were conducted at The University of Melbourne, Australia. Artificial feed was supplied ad libitum to the fed group, and no feed was supplied to the starved group. A modified metabolite extraction protocol using deuterated solvents was developed for 1H-NMR-based metabolite profiling of digestive gland in response to the short-term feeding/starvation experiment, to avoid lyophilisation prior to biochemical analysis. PLS-DA revealed that fed and starved abalone are metabolically distinct from each other after 28 and 56 days. After 28 days, the fed group was defined by an increase in arginine, glucose, glutamate, glycine, inosine and uracil (P < 0.05), and the starved group was defined by an increase in N,N-dimethylglycine. After 56 days, the fed group still displayed increased glucose (P < 0.05), while N,N-dimethylglycine remained elevated in the starved group (P < 0.05). Arginine and glycogen were all higher at 28 days compared to 56 days, suggesting decreased anaerobic energy production at the later time point. Only glucose and N,N-dimethylglycine were significantly different between the fed and starved groups after 56 days, suggesting that abalone had not acclimatised to the starvation treatment after 28 days. These results infer N,N-dimethylglycine is a robust marker for short-term starvation in abalone. 1H-NMR was also conducted on the artificial feed and starved abalone faecal matter, revealing the biochemical differences between them and digestive gland tissue. These methodology and results will facilitate a deeper understanding of the nutritional and physiological requirements of abalone in an aquaculture setting.

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Abbreviations

ANOVA:

Analysis of variance

ATP:

Adenosine triphosphate

DIPSI:

Decoupling in presence of scalar interaction

DSS:

2,2-Dimethyl-2-silapentane-5-sulphonic acid

FID:

Free induction decay

mt:

Metric tonnes

NMR:

Nuclear magnetic resonance spectroscopy

NOESY:

Nuclear overhauser effect spectroscopy

PLS-DA:

Partial least-squares discriminant analysis

1H:

Proton

QC:

Quality control

SD:

Standard deviation

SEM:

Standard error of the mean

TOCSY:

Total correlation spectroscopy

TPPI:

Time-proportional phase incrementation

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Acknowledgments

We wish to thank John Ahern (School of BioSciences, The University of Melbourne) for maintaining the aquaculture facility used in this experiment. We thank Jade Tiger Abalone, part of the Craig Mostyn Group, for providing the abalone used in this study.

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Authors and Affiliations

  1. School of BioSciences, The University of Melbourne, Royal Parade, Parkville, VIC, 3010, Australia

    John R. Sheedy, Sébastien Lachambre, David K. Gardner & Robert W. Day

  2. France Haliotis, Kérazan Lilia, 29880, Plouguerneau, France

    Sébastien Lachambre

  3. IUEM-UBO, UMR CNRS 6539, Technopôle Brest-Iroise, Place Nicolas Copernic, 29280, Plouzané, France

    Sébastien Lachambre

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  1. John R. Sheedy
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  2. Sébastien Lachambre
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Correspondence to John R. Sheedy.

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Sheedy, J.R., Lachambre, S., Gardner, D.K. et al. 1H-NMR metabolite profiling of abalone digestive gland in response to short-term starvation. Aquacult Int 24, 503–521 (2016). https://doi.org/10.1007/s10499-015-9941-4

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