Abstract
The susceptibility of two species of freshwater prawn, Macrobrachium malcolmsonii (17.7 ± 1.85 g, 9.5 ± 0.50 cm) and Macrobrachium rude (4.14 ± 0.95 g, 4.1 ± 0.56 cm), to Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) was tested. These two species were artificially infected with MrNV and XSV by oral and intramuscular routes using viral inoculum prepared from white tail disease—infected post-larvae of M. rosenbergii. No mortality or clinical symptoms were observed in the experimentally infected M. rude and M. malcolmsonii. The data of experimental infection indicate that MrNV and XSV failed to cause the mortality in these two species of freshwater prawn. But RT-PCR analysis of different organs of experimentally infected prawns showed the presence of MrNV and XSV in all the organs analyzed. Further, the viral inoculum prepared from experimentally infected M. malcolmsonii and M. rude caused 100 % mortality in post-larvae of M. rosenbergii (43.0 ± 2 mg, 1.34 ± 0.01 cm, PL stage 10). These results indicate the possibility of M. malcolmsonii and M. rude acting as reservoir for MrNV and XSV and maintaining their virulence in their tissues.
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References
Arcier JM, Herman F, Lightner DV, Redman R, Mari J, Bonami JR (1999) A viral disease associated with mortalities in hatchery-reared postlarvae of the giant freshwater prawn Macrobrachium rosenbergii. Dis Aquat Org 38:177–181
Chen LL, Lo CF, Chiu YL, Chang CF, Kou GH (2000) Natural and experimental infection of white spot syndrome virus WSSV in benthic larvae of mud crab Scylla serrata. Dis Aquat Org 40:157–161
FAO (1998) Aquaculture News Letter, 50
Holthuis LB (1980) FAO Fisheries Synopsis No. 125, vol. 1
Ravi M, Nazeer Basha A, Sarathi M, Rosa Idalia HH, Sri Widada J, Bonami JR, Sahul Hameed AS (2009) Studies on the occurrence of white tail disease (WTD) caused by MrNV and XSV in hatchery-reared post-larvae of Penaeus indicus and P. monodon. Aquaculture 292:117–120
Sahul Hameed AS, Yoganandhan K, Sri Widada J, Bonami JR (2004a) Studies on the occurrence and RT-PCR detection of Macrobrachium rosenbergii nodavirus and extra small virus-like particles associated with white tail disease of Macrobrachium rosenbergii in India. Aquaculture 238:127–133
Sahul Hameed AS, Yoganandhan K, Sri Widada J, Bonami JR (2004b) Experimental transmission and tissue tropism of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus like-particles in Macrobrachium rosenbergii. Dis Aquat Org 62:191–196
Senapin S, Jaengsanong C, Phiwsaiya K, Prasertsri S, Laisutisan K, Chuchird N, Limusuwan C, Flegel TW (2012) Infection of MrNV (Macrobrachium rosenbergii nodavirus) in cultivated whiteleg shrimp Penaeus vannamei in Asia. Aquaculture 338:41–46
Sri Widada J, Bonami JR (2004) Characteristics of the monocistronic genome of extra small virus, a virus-like particle associated with Macrobrachium rosenbergii nodavirus: possible candidate for a new species of satellite virus. J Gen Virol 85:643–646
Sri Widada J, Durand S, Cambournac I, Qian D, Shi Z, Dejonghe E, Richard V, Bonami JR (2003) Genome-based detection methods of Macrobrachium rosenbergii nodavirus, a pathogen of the giant freshwater prawn, Macrobrachium rosenbergii: dot-blot, in situ hybridization and RT-PCR. J Fish Dis 26:583–590
Sudhakaran R, Syed Musthaq S, Haribabu P, Mukherjee SC, Gopal C, Sahul Hameed AS (2006a) Experimental transmission of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) in three species of marine shrimp (Penaeus indicus, Penaeus japonicus and Penaeus monodon). Aquaculture 257:136–141
Sudhakaran R, Yoganandhan K, Ishaq Ahmed VP, Sahul Hameed AS (2006b) Artemia as a possible vector for Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus transmission to Macrobrachium rosenbergii post-larvae. Dis Aquat Org 70:161–166
Sudhakaran R, Haribabu P, Rajesh Kumar S, Sarathi M, Ishaq Ahmed VP, Sarath Babu V, Venkatesan C, Sahul Hameed AS (2008) Natural aquatic insect carriers of Macrobrachium rosenbergii noda virus (MrNV) and extra small virus (XSV). Dis Aquat Org 79:141–145
Venegas CA, Nonaka L, Mushiake K, Nishizawa T, Muroga K (1999) Pathogenicity of penaeid rod-shaped DNA virus PRDV to kuruma prawn at different development stages. Fish Pathol 170:179–194
Acknowledgments
The authors are grateful to the Management of C. Abdul Hakeem College, Melvisharam, India, for providing the facilities to carry out this work. This study was funded by Department of Biotechnology, New Delhi, India.
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Ravi, M., Sahul Hameed, A.S. Experimental transmission of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) in Macrobrachium malcolmsonii and Macrobrachium rude . Aquacult Int 23, 195–201 (2015). https://doi.org/10.1007/s10499-014-9808-0
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DOI: https://doi.org/10.1007/s10499-014-9808-0