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Effect of temperature decrease on hemocyte apoptosis of the white shrimp Litopenaeus vannamei

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Abstract

This study investigated the effect of continuous temperature decrease on hemocyte apoptosis of the white shrimp Litopenaeus vannamei. In the stress group, water temperature decreased from 26 to 17 °C at a rate of 1 °C/h. Shrimp kept at 26 ± 0.5 °C were used as control group. Total hemocyte count (THC), reactive oxygen species (ROS) production, cytoplasmic free-Ca2+ (CF-Ca2+) concentration, mitochondrial membrane potential (MMP), apoptotic cell ratio, and caspase-3 activity of L. vannamei hemocytes were determined when water temperature decreased to 23, 20, and 17 °C, respectively. Increased ROS production in hemocytes was observed when water temperature decreased to 20 and 17 °C. Decreased THC and cellular MMP, increased CF-Ca2+ concentration, apoptotic cell ratio, and caspase-3 activity were shown when water temperature decreased to 17 °C. These results indicate that water temperature decrease can induce oxidative stress on shrimp hemocytes and then cause mitochondria and caspase-3 mediated hemocyte apoptosis and THC reduction, when water temperature decreased to an unconformable level.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (31302164 and 30671628), China Postdoctoral Science Foundation (2012M511829), Guangdong Provincial Natural Science Foundation (S2011020003256 and S2012040008093), Scientific and Technological Planning Project of Guangdong Province (2011B020307010 and 2012B020307004), Project of Guangdong Provincial Oceanic and Fishery Administration (A200901B06), and the Scientific and Technological Planning Project of Guangzhou City (11A82090870).

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Authors and Affiliations

  1. Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, Guangzhou, 510631, People’s Republic of China

    Bin Li, Jian-An Xian, Hui Guo, An-Li Wang, Yu-Tao Miao, Jian-Min Ye, Chao-Xia Ye & Shao-An Liao

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  1. Bin Li
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  2. Jian-An Xian
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  3. Hui Guo
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  4. An-Li Wang
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  5. Yu-Tao Miao
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  6. Jian-Min Ye
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  7. Chao-Xia Ye
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  8. Shao-An Liao
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Correspondence to An-Li Wang.

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Bin Li and Jian-An Xian contributed equally to this work.

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Li, B., Xian, JA., Guo, H. et al. Effect of temperature decrease on hemocyte apoptosis of the white shrimp Litopenaeus vannamei . Aquacult Int 22, 761–774 (2014). https://doi.org/10.1007/s10499-013-9704-z

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  • DOI: https://doi.org/10.1007/s10499-013-9704-z

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