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Effect of geographic origin, temperature and timing of broodstock collection on conditioning, spawning success and larval viability of Ruditapes decussatus (Linné, 1758)

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Abstract

Culture of Ruditapes decussatus is clearly limited by the availability of seed, as this production proceeds almost exclusively from natural recruitment. Artificial spawning and larval rearing programs could provide an alternative source of spat. This study was designed to evaluate the effect of different conditioning temperatures on the broodstock maturation, spawning success and larval viability of two geographically (north and south of the Iberian Peninsula) distinct populations of European clam (R. decussatus) collected at different periods of the year in order to create “optimal” artificial spawning and larval rearing programs. Two batches of clams from each population were collected in October and February, and conditioned at 18 ± 1°C, 20 ± 1°C and 22 ± 1°C. Of the three variables analysed the timing of broodstock collection was the most determining factor for gametogenic development, spawning and larval rearing. Geographic origin and conditioning temperature also greatly affected the spawning. The results also showed that the February conditioning was more effective than October and that the best conditioning temperatures were 20 ± 1°C and 22 ± 1°C for the northern and southern populations, respectively. These results suggest that the efficient conditioning temperature for each population of the same species is related to the seasonal temperature regime from their geographic origin. Larval viability and growth performance seemed to be independent of the broodstock conditioning.

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Abbreviations

AFDW:

Ash free dry weight

ANOVA:

Analysis of variance

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Acknowledgements

The authors are grateful to Dr. M. Castro for reviewing and improving the statistical treatments and to J. White for revising the English. Gratitude is also extended to António Machado, who was in charge of the broodstock.

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Authors and Affiliations

  1. Instituto de Recursos Biológicos, I.P./L-IPIMAR, Av. 5 de Outubro, 8700-305, Olhão, Portugal

    Domitília Matias, Sandra Joaquim, Alexandra Leitão & Clara Massapina

  2. Instituto de Biotecnologia e Bioengenharia, Centro de Genética e Biotecnologia (IBB/CGB-UTAD), 5000-911, Vila Real, Portugal

    Alexandra Leitão

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  1. Domitília Matias
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  2. Sandra Joaquim
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Correspondence to Domitília Matias.

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Matias, D., Joaquim, S., Leitão, A. et al. Effect of geographic origin, temperature and timing of broodstock collection on conditioning, spawning success and larval viability of Ruditapes decussatus (Linné, 1758). Aquacult Int 17, 257–271 (2009). https://doi.org/10.1007/s10499-008-9197-3

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