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MS222 does not induce primary DNA damage in fish

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Abstract

The genotoxicity of the anaesthetic MS222 (tricaine) was analysed in fish under both in vivo and in vitro conditions. Based on results of the single cell gel/Comet assay, MS222 had no direct genotoxic effect on the experimental fish, indicating that MS222 does not induce primary DNA damage. These results suggest that the use of this important anaesthetic in aquaculture can be considered to be safe in terms of genotoxicity.

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Acknowledgements

The authors thank Mr. A.C.B. Tardivo for assistance during the experiments.

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Authors and Affiliations

  1. Departamento de Fisiologia, Instituto de Biociências, UNESP, Rubião Jr. s/n, 18618-000, Botucatu, SP, Brazil

    R. E. Barreto, V. C. Raymundi & G. L. Volpato

  2. Unité de Biologie des Interactions Hôte-Parasite, Institut Pasteur, Paris, France

    A. M. M. C. Gontijo

  3. TOXICAN – Núcleo de Avaliação Toxicogenética e Cancerígena, Departamento de Patologia, Faculdade de Medicina, UNESP, Botucatu, SP, Brazil

    R. O. Alves-de-Lima, D. Pinhal, V. A. V. Reyes & D. M. F. Salvadori

Authors
  1. R. E. Barreto
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  2. A. M. M. C. Gontijo
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  3. R. O. Alves-de-Lima
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  4. V. C. Raymundi
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  5. D. Pinhal
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  6. V. A. V. Reyes
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  7. G. L. Volpato
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  8. D. M. F. Salvadori
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Corresponding author

Correspondence to R. E. Barreto.

Additional information

R. E. Barreto and A. M. M. C. Gontijo contributed equally to the study.

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Barreto, R.E., Gontijo, A.M.M.C., Alves-de-Lima, R.O. et al. MS222 does not induce primary DNA damage in fish. Aquacult Int 15, 163–168 (2007). https://doi.org/10.1007/s10499-007-9073-6

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  • DOI: https://doi.org/10.1007/s10499-007-9073-6

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