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Circ_0000523 regulates miR-1184/COL1A1/PI3K/Akt pathway to promote nasopharyngeal carcinoma progression

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Abstract

Background

The present study is to investigate the biological functions and mechanisms of circular RNA_0000523 (circ_0000523) in nasopharyngeal carcinoma (NPC).

Methods

Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was conducted to examine the expression levels of circ_0000523 and microRNA-1184 (miR-1184) in NPC tissues and cells. Collagen type 1 alpha 1 chain (COL1A1) expression was assessed by qRT-PCR and immunohistochemistry (IHC) assay. Cell proliferation, cell cycle progression, migration and invasion were examined by cell counting kit-8 (CCK-8), 5-bromo-2’-deoxyuridine (BrdU), flow cytometry and Transwell assays. Xenograft nude mouse models were used to investigate the metastatic potential of NPC cells in vivo. The binding relationships between circ_0000523 and miR-1184, and between miR-1184 and COL1A1 were detected by dual-luciferase reporter gene assay. The protein expressions of COL1A1, phosphatidylinositol 3-kinase (p85), phosphorylated (p)-p85, protein kinase B (Akt) and p-Akt were detected through Western blot. The DAVID database was used for the enrichment analysis of the potential targets of miR-1184.

Results

Circ_0000523 and COL1A1 mRNA expressions were significantly increased in NPC tissues and cell lines. Circ_0000523 overexpression promoted NPC cell proliferation and accelerated cell cycle progression, whereas miR-1184 overexpression reversed these effects; circ_0000523 knockdown suppressed NPC cell proliferation and induced cell cycle arrest, while miR-1184 inhibition counteracted these effects. MiR-1184 was the downstream target of circ_0000523, and COL1A1 was the target gene of miR-1184 and could be positively modulated by circ_0000523. COL1A1 overexpression increased the expression levels of p-p85 and p-Akt, whereas knocking down COL1A1 repressed their expressions.

Conclusions

Circ_0000523 facilitates NPC progression through regulating the miR-1184/COL1A1 axis.

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Authors and Affiliations

  1. Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital, Central South University, Renmin Middle Road No. 139, 410011, Changsha, Hunan Province, China

    Peiying Huang, Mengmeng Li, Qinglai Tang, Kang Jiang & Yuchao Luo

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  1. Peiying Huang
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  2. Mengmeng Li
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  3. Qinglai Tang
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  4. Kang Jiang
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  5. Yuchao Luo
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Correspondence to Mengmeng Li.

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Huang, P., Li, M., Tang, Q. et al. Circ_0000523 regulates miR-1184/COL1A1/PI3K/Akt pathway to promote nasopharyngeal carcinoma progression. Apoptosis 27, 751–761 (2022). https://doi.org/10.1007/s10495-022-01743-y

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  • DOI: https://doi.org/10.1007/s10495-022-01743-y

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