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Local hyperthermia induces apoptosis of keratinocytes in both normal skin and condyloma acuminata via different pathways

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Abstract

Local hyperthermia has been successfully used in the treatment of viral warts. However, the mechanism of action of hyperthermia has largely remained unclear. In this study we evaluated the effect of local hyperthermia on the induction of apoptosis in human keratinocytes, and expression of apoptosis-related genes in both condyloma acuminata (CA) and normal skin. The study showed that higher hyperthermia increased the number of apoptotic keratinocytes in CA and normal skin. The temperature-dependent increased expression of Fas and Bax were observed in both CA and normal skin. In contrast, the expression of Bcl-2 in CA was decreased at both transcriptional and translational levels. Furthermore, the transcriptional expression of DR4 and DR5 were increased in a temperature-dependent manner in CA, but not in normal skin. These results suggest that different mechanisms of action might be involved in hyperthermia induced apoptosis in CA and normal skin.

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Abbreviations

CA:

Condyloma acuminata

HPV:

Human papillomavirus

TNF:

Tumor necrosis factor

OPG:

Osteoprotegerin

DD:

Death domains

DR:

Death receptor

PBS:

Phosphate buffered solution

TRAIL:

Tumor necrosis factor-related apoptosis-inducing ligand

TUNEL:

Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling

IHC:

Immunohistochemistry

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Acknowledgments

This study was supported by National Science Foundation (30740082), Program for New Century Excellent Talents in University (NCEP-04-0287) and Program for Changjiang Scholars and Innovative Research Team in University (IRT0760), Ministry of Education.

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Dermatology, No. 1 Hospital of China Medical University, 110001, Shenyang, China

    Xiaoqin Wang, Xing-Hua Gao, Xiaodong Li, Yuxiao Hong, Ruiqun Qi, Hong-Duo Chen, Li Zhang & Huachen Wei

  2. Department of Dermatology, Shengjing Hospital of China Medical University, 110004, Shenyang, China

    Xiaoqin Wang

  3. Department of Dermatology, Mount Sinai Medical Center, New York, NY, 10029, USA

    Huachen Wei

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  1. Xiaoqin Wang
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Correspondence to Xing-Hua Gao.

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Wang, X., Gao, XH., Li, X. et al. Local hyperthermia induces apoptosis of keratinocytes in both normal skin and condyloma acuminata via different pathways. Apoptosis 14, 721–728 (2009). https://doi.org/10.1007/s10495-009-0344-8

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  • DOI: https://doi.org/10.1007/s10495-009-0344-8

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