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Epicatechin protects auditory cells against cisplatin-induced death

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Abstract

Cisplatin, a chemotherapeutic drug that is widely used to treat various cancers, promotes ototoxicity at higher doses. In this study, the effect of epicatechin (EC) on cisplatin-induced hair cell death was investigated in a cochlear organ of Corti-derived cell line, HEI-OC1, and in vivo in zebrafish. Cisplatin promoted apoptosis and altered mitochondrial membrane potential (MMP) in HEI-OC1 cells. EC inhibited cisplatin-induced apoptosis and intracellular reactive oxygen species (ROS) generation. Labeling of zebrafish lateral line hair cells by the fluorescent dye YO-PRO1 was lost upon exposure to cisplatin, and EC protected against this cisplatin-induced loss of labeling in a dose-dependent manner. Scanning and transmission electron micrographs showed that treatment with EC protected against cisplatin-induced loss of kinocilium and stereocilia in zebrafish neuromasts. These results suggest that EC prevents cisplatin-induced ototoxicity by blocking ROS generation and by preventing changes in MMP.

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Acknowledgements

This research was supported by CCRB through the “GRRC” Project of the Gyeonggi Provincial Government, Republic of Korea, and the Korea Research Foundation Grant funded by the Korean government (MOEHRD; KRF-2006-331-E00260).

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Authors and Affiliations

  1. Department of Otolaryngology, School of Medicine, Ajou University, 5 Wonchon-Dong, Yeongtong-Gu, Suwon, 442-749, South Korea

    Chul-Ho Kim, Sung Un Kang, JungHee Pyun, Mi Hye Lee, Hye Sook Hwang & HaNeul Lee

  2. Center for Cell Death Regulating Biodrugs, School of Medicine, Ajou University, Suwon, South Korea

    Chul-Ho Kim, Sung Un Kang, JungHee Pyun, Mi Hye Lee & Hye Sook Hwang

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  1. Chul-Ho Kim
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Correspondence to Chul-Ho Kim.

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Kim, CH., Kang, S.U., Pyun, J. et al. Epicatechin protects auditory cells against cisplatin-induced death. Apoptosis 13, 1184–1194 (2008). https://doi.org/10.1007/s10495-008-0242-5

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