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Cell death pathways in juvenile Batten disease

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Abstract

Apoptosis, Golgi fragmentation and elevated ceramide levels occur in Juvenile Neuronal Ceroid Lipofuscinosis (JNCL) neurons, lymphoblasts and fibroblasts. Our purpose was to examine whether apoptosis is the mechanism of cell death in JNCL. This was tested by analyzing caspase-dependent/independent pathways and autophagy, and caspase effects on ceramide and Golgi fragmentation. zVAD prevented caspase activation, but not all cell death. Inhibiting caspase-8 suppressed caspases more than inhibition of any other caspase. Inhibiting caspase-8/6 was synergistic. zVAD suppressed autophagy. 3-methyladenine suppressed caspase activation less than zVAD did. Blocking autophagy/caspase-8/or-6 was synergistic. Blocking autophagy/caspase-3/or-9 was not. Inhibiting caspase-9/3 suppressed autophagy. Golgi fragmentation was suppressed by zVAD, and blocked by CLN3. CLN3, not zVAD, prevented ceramide elevation. In conclusion: caspase-dependent/independent apoptosis and autophagy occur caspase-dependent pathways initiate autophagy Golgi fragmentation results from apoptosis ceramide elevation is independent of caspases, and CLN3 blocks all cell death, prevents Golgi fragmentation and elevation of ceramide in JNCL.

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Authors and Affiliations

  1. Departments of Pediatrics and Neurobiology, Duke University Medical Center, MSRB, Research Drive, Box 2604, Durham, NC, 27710

    D. A. Persaud-Sawin & R-M. N. Boustany

  2. Departments of Pediatrics and Neurobiology, DUMC, MSRB Building, Room 281 B, Research Drive, Durham, NC, 27710

    R-M. N. Boustany

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  1. D. A. Persaud-Sawin
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Persaud-Sawin, D.A., Boustany, RM.N. Cell death pathways in juvenile Batten disease. Apoptosis 10, 973–985 (2005). https://doi.org/10.1007/s10495-005-0733-6

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