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Laboratory assessment of acaricidal activity of Cymbopogon winterianus, Vitex negundo and Withania somnifera extracts against deltamethrin resistant Hyalomma anatolicum

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Abstract

Larval packet test was used for detection of resistance levels against cypermethrin and deltamethrin, the most commonly used synthetic pyrethroids, in the multi-host tick Hyalomma anatolicum collected from district Moga, Punjab (India). Results indicated the presence of level I resistance against deltamethrin (RF = 2.81), whereas the tick isolate was susceptible to cypermethrin (RF = 0.2). The aqueous and ethanolic extracts of leaves of Cymbopogon winterianus, Vitex negundo and Withania somnifera along with roots of Vitex negundo were assessed for their acaricidal activity against the larvae of deltamethrin resistant H. anatolicum. The efficacy was assessed by measuring per cent larval mortality and determination of LC50 values. The various ethanolic extracts produced a concentration dependent increase in larval tick mortality, whereas the aqueous extracts exhibited a much lower mortality. The highest mortality (93.7 ± 0.66 %) was observed at the 5.0 % concentration of ethanolic extract of leaves of C. winterianus and the lowest LC50 value (0.011 %) was recorded for ethanolic extracts of leaves of V. negundo. The results indicated that these plant extracts have potential to be developed as herbal acaricides.

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Acknowledgments

Authors are thankful to the Director of Research, GADVASU, Ludhiana for providing facilities to carry out the research work.

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Authors and Affiliations

  1. Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, India

    Nirbhay Kumar Singh,  Jyoti, Abhijit Nandi & Harkirat Singh

  2. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, India

    Bhaskar Vemu & V. K. Dumka

  3. Department of Agronomy, Punjab Agricultural University, Ludhiana, 141004, India

    Rajender Kumar

Authors
  1. Nirbhay Kumar Singh
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  2. Jyoti
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  3. Bhaskar Vemu
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  4. Abhijit Nandi
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  5. Harkirat Singh
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  6. Rajender Kumar
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  7. V. K. Dumka
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Correspondence to Nirbhay Kumar Singh.

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Singh, N.K., Jyoti, Vemu, B. et al. Laboratory assessment of acaricidal activity of Cymbopogon winterianus, Vitex negundo and Withania somnifera extracts against deltamethrin resistant Hyalomma anatolicum . Exp Appl Acarol 63, 423–430 (2014). https://doi.org/10.1007/s10493-014-9791-1

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  • DOI: https://doi.org/10.1007/s10493-014-9791-1

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