Abstract
Estimating the spatial and temporal variation in tick abundance is of great economical and ecological importance. Entire-blanket dragging is the most widely used method to sample free-living ixodid ticks. However, this technique is not equally efficient in different vegetation types. The height and structure of the vegetation under study will not only determine the likelihood of a tick-blanket contact, but will also determine the rate of dislodgement. The purpose of this study was therefore to determine whether the alternative strip-blanket is more effectively in picking up ticks than the standard entire-blanket. Sampling was carried out in four forest understory vegetation types that differed in height and structure on five collection dates between April and September 2008. A total of 8,068 Ixodes ricinus ticks was collected (778 adults, 1,920 nymphs, and 5,370 larvae). The highest numbers of ticks were collected along the forest trails, where the dominant vegetation consisted of short grasses. The lowest numbers of ticks were collected in bracken-fern-dominated sites, where the vegetation seriously hampered tick sampling. Surprisingly, in each vegetation type, significantly more nymphs and adults were collected using the entire-blanket. However, the strip-blanket was more effectively in collecting larvae, especially in dense and tall vegetation.
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References
Bacon RM, Kugeler KJ, Mead PS (2008) Surveillance for Lyme disease: United States, 1992–2006. MMWR Morb Mortal Wkly Rep 57:1–9
Falco RC, Fish D (1992) A comparison of methods for sampling the deer tick, Ixodes dammini, in a Lyme disease endemic area. Exp Appl Acarol 14:165–173
Falco RC, McKenna DF, Daniels TJ, Nadelman RB, Nowakowski J, Fish D, Wormser GP (1999) Temporal relation between Ixodes scapularis abundance and risk for Lyme disease associated with erythema migrans. Am J Epidemiol 149:771–776
Ginsberg HS, Ewing CP (1989) Comparison of flagging, walking, trapping, and collecting from hosts as sampling methods for northern deer ticks, Ixodes dammini, and lone-star ticks, Amblyomma americanum (Acari: Ixodidae). Exp Appl Acarol 7:313–322
Gray JS (1985) A carbon dioxide trap for prolonged sampling of Ixodes ricinus L. populations. Exp Appl Acarol 1:35–44
Gray JS, Lohan G (1982) The development of a sampling method for the tick Ixodes ricinus and its use in a redwater fever area. Ann Appl Biol 101:421–427
Li X, Dunley JE (1998) Optimal sampling and spatial distribution of Ixodes pacificus, Dermacentor occidentalis and Dermacentor variabilis ticks (Acari: Ixodidae). Exp Appl Acarol 22:233–248
Liang K-Y, Zeger SL (1986) Longitudinal data analysis using generalized linear models. Biometrika 73:13–22
Mather TN, Nicholson MC, Donnelly EF, Matyas BT (1996) Entomologic index for human risk of Lyme disease. Am J Epidemiol 144:1066–1069
Mejlon HA, Jaenson TGT (1997) Questing behaviour of Ixodes ricinus ticks (Acari: Ixodidae). Exp Appl Acarol 21:747–754
Milne A (1943) The comparison of sheep-tick populations (Ixodes ricinus L.). Ann Appl Biol 30:240–250
Needham GR, Teel PD (1991) Off-host physiological ecology of Ixodid ticks. Annu Rev Entomol 36:659–681
Piesman J, Gern L (2004) Lyme borreliosis in Europe and North America. Parasitology 129:S191–S220
Randolph SE, Storey K (1999) Impact of microclimate on immature tick-rodent host interactions (Acari: Ixodidae): implications for parasite transmission. J Med Entomol 36:741–748
Ruiz-Fons F, Gilbert L (2010) The role of deer as vehicles to move ticks, Ixodes ricinus, between contrasting habitats. Int J Parasitol 40:1013–1020
Stafford KC III, Cartter ML, Magnarelli LA, Ertel S-H, Mshar PA (1998) Temporal correlations between tick abundance and prevalence of ticks infected with Borrelia burgdorferi and increasing incidence of Lyme disease. J Clin Microbiol 36:1240–1244
Sutherst RW, Wharton RH, Utech KBW (1978) Guide to studies on tick ecology. Division of Entomology, Commonwealth Scientific and Industrial Research Organization, Technical Paper No. 14, 59 pp
Tälleklint L, Jaenson TGT (1994) Transmission of Borrelia burgdorferi s.l. from mammal reservoirs to the primary vector of Lyme borreliosis, Ixodes ricinus (Acari: Ixodidae), in Sweden. J Med Entomol 31:880–886
WHO (2004) The vector-borne human infections of Europe: their distribution and burden on public health. World Health Organization Regional Office for Europe, Copenhagen, 144 pp
Acknowledgments
The authors thank Marijn Tormans, Kris Ceunen, Karen Wuyts, and Miguel Lyssens-Danneboom for assistance with fieldwork. We are also grateful to Natuurpunt vzw for the permission to work in the nature reserve. This research was funded by IWT-Flanders, the Institute for the Promotion of Innovation by Science and Technology in Flanders.
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Tack, W., Madder, M., De Frenne, P. et al. The effects of sampling method and vegetation type on the estimated abundance of Ixodes ricinus ticks in forests. Exp Appl Acarol 54, 285–292 (2011). https://doi.org/10.1007/s10493-011-9444-6
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DOI: https://doi.org/10.1007/s10493-011-9444-6