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Ticks Parasitizing Wild Birds in Portugal: Detection of Rickettsia aeschlimannii, R. helvetica and R. massiliae

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Abstract

From January 2002 to December 2004, 152 ticks were collected from 40 wild birds recovered in Santo André Natural Reserve and Monsanto Forestal Park, Portugal mainland. Five ticks species were identified from 22 species of birds, and new host record were provided for some species. In addition, 32 (21%) ticks were screened by PCR to detect infections with agents belonging to order Rickettsiales: Anaplasma phagocytophilum, Ehrlichia chaffeensis, and Rickettsia spp. PCR amplicons were obtained in 5 (15.6%) tick samples. Rickettsia DNA exhibiting gltA sequences similar to those of Rickettsia aeschilimannii, R. helvetica and R. massiliae were identified in Hyalomma marginatum, Ixodes ventalloi and in Rhipicephalus turanicus, respectively. This is the first report of rickettsiae infections in ticks collected from wild birds in Portugal. Giving the results presented above wild birds play an important role in the maintenance and dissemination of several tick species and associated rickettsiae.

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References

  • Alekseev AN, Dubinina HV, Semenov AV, Bolshakov CV (2001) Evidence of ehrlichiosis agents found in ticks (Acari: Ixodidae) collected from migratory birds. J Med Entomol 38:471–474

    Article  PubMed  CAS  Google Scholar 

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990). Basic local alignment search tool. J␣Mol Biol 215:403–410

    PubMed  CAS  Google Scholar 

  • Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller M, Lipman FJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  PubMed  CAS  Google Scholar 

  • Bacellar F (1999) Ticks and Rickettsiae in Portugal. In: Raoult D, Brouqui P (eds) Rickettsiae and Rickettsial Diseases. Elsevier, Paris, pp. 103–109

    Google Scholar 

  • Bjoersdorf A, Bergstrom S, Massung RF, Haemig PD, Olsen B (2001) Ehrlichia-infected ticks on migrating birds. Emerging Infect Dis 7:877–879

    Article  Google Scholar 

  • Bown KJ, Begon M, Bennett M, Woldehiwet Z, Ogden NH (2003) Seasonal dynamics of Anaplasma phagocytophila in a rodent-tick (Ixodes trianguliceps) system, United Kingdom. Emerg Infect Dis 9(1):63–70

    PubMed  Google Scholar 

  • Caeiro V (1999) General review of tick species present in Portugal. Parasitol 41:11–15

    Google Scholar 

  • Caspersen K, Park J-H, Patil S, Dumler JS (2002) Genetic variability and stability of Anaplasma phagocytophila msp2 (p44). Infect Immun 70(3):1230–1234

    Article  PubMed  CAS  Google Scholar 

  • CEMPA/ ICN (1995) A anilhagem de aves na investigação científica e na gestão ambiental. Centro de Estudos de Migração e Protecção das Aves (CEMPA), Edição Portuguesa do Instituto da Conservação da Natureza (ICN), Lisboa, pp 1–24

  • Cordas T, Aeschlimann A, Morel PC (1993) Étude morphologique des ixodidae s. str. (Schulze, 1937) de Suisse au microscope Électronique à balayage. Acarologia 34(1):21–46

    Google Scholar 

  • Daniels TJ, Battaly GR, Liveris D, Falco RC, Schwartz I (2002) Avian reservois of the agent of human granulocytic ehrlichiosis? Emerging Infect Dis 8:1524–1525

    PubMed  Google Scholar 

  • Dawson JE, Biggie KL, Warner CK, Cookson K, Jenkins S, Levine JF, Olson JG (1996) Polymerase chain reaction evidence of Ehrlichia chaffeensis, an etiologic agent of human ehrlichiosis, in dogs from southeast Virginia. AJVR 57(8):1175–1179

    CAS  Google Scholar 

  • Dias JATS (1994) As carraças (Acarina-Ixodoidea) da Península Ibérica. Algumas considerações sobre a sua Biogeografia e relacionamento com a ixodofauna Afropaleártica e Afrotropical. Instituto de Investigação Científica Tropical, Estudos Ensaios e Documentos, Lisboa, pp 1–163

  • Estrada-Peña A, Santos-Silva M (2005) The distribution of ticks (Acari:Ixodidae) of domestic livestock in Portugal. Exp App Acarol 36:233–246

    Article  Google Scholar 

  • Gil Collado J (1936) Acaros Ixodoideos de Cataluña y Baleares. Trab Mus Cienc Nat, Ser Ent 11:1–8

    Google Scholar 

  • Gilot B, Marjolet M (1982) Contribution à l’étude du parasitisme humain par les tiques (Ixodidae et Argasidae), plus particulièrement dans le sud-est de la France. Méd Maladies Infect 12(6):340–351

    Article  Google Scholar 

  • Giustina V, Serafino M, Rolain J-M, Raoult D (2006) Rickettsia massiliae human isolation. Emerg Infect Dis 12(1):174–175

    Google Scholar 

  • Hoogstraal H (1956) African Ixodoidea. 1. Ticks of Sudan. Bureau of Medicine and Surgery, US Navy, pp 1–1101

  • Hoogstraal H (1961) Migrating birds and their ectoparasites in relation to disease. East Afric Med J 38(5):221–226

    CAS  Google Scholar 

  • Hoogstraal H, Kaiser M (1961) Ticks from European-Asiatic birds migrating through Egypt into Africa. Science 3448(133):227–278

    Google Scholar 

  • Hoogstraal H, Kaiser M, Traylor M, Gaber S, Guindy E (1961) Ticks (Ixodoidea) on birds migrating from Africa to Europe and Asia. Bull Wld Hlth Org 24:197–212

    CAS  Google Scholar 

  • Hoogstraal H, Kaiser M, Traylor M, Guindy E, Gaber S (1963) Ticks (Ixodidae) on birds migrating from Europe and Asia to Africa, 1959–61. Bull Wld Hlth Org 28:235–262

    CAS  Google Scholar 

  • Hoogstraal H, Aeschlimann A (1982) Tick-host specificity. Bull Soc Entomol Suisse 55:5–32

    Google Scholar 

  • Hubalek Z (2004) An annotated checklist of pathogenic microorganisms associated with migratory birds. J Wildlife Dis 40(4):639–659

    Google Scholar 

  • Nilsson K, Lindquist O, Pahlson C (1999) Association of Rickettsia helvetica with chronic perimyocarditis in sudden death. Lancet 354:1169–1172

    Article  PubMed  CAS  Google Scholar 

  • Osacar-Jimenez JJ, Estrada-Peña A, Lucientes-Curdi J (1998) Ticks (Acarina: Ixodidae) of wild birds in the Ebro middle basin (North-east Spain). Acarologia 39(1): 23–31

    Google Scholar 

  • Papadopoulos B, Humair PF, Aeschlimann A, Vaucher C, Buttiker W (2001) Ticks on birds in Switzerland. Acarology 42(1):3–19

    Google Scholar 

  • Raoult D, Fournier PE, Abboud P, Caron F (2002) First documented human Rickettsia aeschlimannii infection. Emerg Infect Dis 8(7):748–749

    PubMed  Google Scholar 

  • Regnery RL, Spruill CL, Plikaytis BD (1991) Genotypic identification of rickettsiae and estimation of intraspecies sequences divergence for portions of two rickettsial genes. J Bacteriol 173(5):1576–1589

    PubMed  CAS  Google Scholar 

  • Santos AS, Santos-Silva MM, Almeida VC, Bacellar F, Dumler JS (2004) Detection of Anaplasma phagocytophilum DNA in Ixodes ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, Mainland Portugal. Emerg Infect Dis 10:1643–1648

    PubMed  CAS  Google Scholar 

  • Schouls LM, Van de Pol I, Rijpkema SGT, Schot CS (1999) Detection and identification of Ehrlichia, Borrelia burgdorferi sensu lato and Bartonella species in Dutch Ixodes ricinus ticks. J␣Clin Microbiol 37(7):2215–2222

    PubMed  CAS  Google Scholar 

  • Silva MM, Formosinho P, Melo P, Santos A, Filipe AR (2001) Ixodídeos (Acari: Ixodidae) parasitas de aves silváticas em Portugal. Rev Port Cienc Vet 96(540):197–199

    Google Scholar 

  • Somov GP, Soldatov GM (1964) On the role of birds in circulation of tick-borne spotted typhus in nature. Zh Mikrobiol Epidemiol Immunobiol 1:126–129 (In Russian)

    Google Scholar 

  • Syrucek L, Raska K (1956) Q fever in domestic and wild birds. Bull WHO 15:329–337

    PubMed  Google Scholar 

Download references

Acknowledgements

We thank ICN technicians for their valuable contribution in the fieldwork. We also thank Liliana Vitorino for contribution in sequencing alignment and National Institute of Health for supporting this research.

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Authors and Affiliations

  1. Centro de Estudos de Vectores e Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Avenida da Liberdade 5, 2965-575, Águas de Moura, Portugal

    Maria Margarida Santos-Silva, Rita Sousa, Ana Sofia Santos & Fátima Bacellar

  2. Centro de Recuperação de Animais Silvestres/Parque Florestal Monsanto, Câmara Municipal de Lisboa, Lisboa, Portugal

    Pedro Melo

  3. Instituto da Conservação da Natureza, Lisboa, Portugal

    Victor Encarnação

Authors
  1. Maria Margarida Santos-Silva
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  2. Rita Sousa
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  3. Ana Sofia Santos
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  4. Pedro Melo
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  5. Victor Encarnação
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  6. Fátima Bacellar
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Correspondence to Maria Margarida Santos-Silva.

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Santos-Silva, M.M., Sousa, R., Santos, A.S. et al. Ticks Parasitizing Wild Birds in Portugal: Detection of Rickettsia aeschlimannii, R. helvetica and R. massiliae . Exp Appl Acarol 39, 331–338 (2006). https://doi.org/10.1007/s10493-006-9008-3

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  • DOI: https://doi.org/10.1007/s10493-006-9008-3

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