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Identification of astigmatid mites using the second internal transcribed spacer (ITS2) region and its application for phylogenetic study

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Abstract

The second internal transcribed spacer (ITS2) of nuclear ribosomal DNA from 73 specimens of Astigmata was analyzed by PCR amplification and DNA sequencing. The length of the ITS2 region varied from 282 to 592 bp. The interspecific variation based on consensus sequences was more than 4.1%, while the intraspecific or intra-individual variation was from 0 to 5.7%. The variation between geographically separated populations (0–3.2%) was almost the same as the variation within strains. The sequences of the ITS2 region of Astigmata were concluded to be species-specific. The phylogenetic tree inferred from the ITS2 region supported Zachvatkin’s morphological classification in the subfamily Rhizoglyphinae. The species-specific ITS2 sequence is useful for the species identification of astigmatid mites and for studying low-level phylogenetic relationships.

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Authors and Affiliations

  1. Laboratory of Chemical Ecology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, 606-8502, Sakyo-ku, Kyoto, Japan

    Koji Noge, Naoki Mori & Yasumasa Kuwahara

  2. Laboratory of Environmental Mycoscience, Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan, 606-8502

    Chihiro Tanaka, Ritsuo Nishida & Mitsuya Tsuda

Authors
  1. Koji Noge
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  2. Naoki Mori
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  3. Chihiro Tanaka
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  4. Ritsuo Nishida
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  5. Mitsuya Tsuda
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  6. Yasumasa Kuwahara
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Correspondence to Koji Noge.

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Chemical Ecology of Astigmatid Mites LXXV

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Noge, K., Mori, N., Tanaka, C. et al. Identification of astigmatid mites using the second internal transcribed spacer (ITS2) region and its application for phylogenetic study. Exp Appl Acarol 35, 29–46 (2005). https://doi.org/10.1007/s10493-004-1953-0

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  • DOI: https://doi.org/10.1007/s10493-004-1953-0

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