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Heminiphilus faecis gen. nov., sp. nov., a member of the family Muribaculaceae, isolated from mouse faeces and emended description of the genus Muribaculum

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Abstract

The novel strain AM35T was isolated from the faeces of C57BL/6 mice. These cells are strictly anaerobic, gram negative, oxidase negative, catalase positive, rod-shaped and non-motile. The strain produced creamy yellowish colonies on brain heart infusion (BHI) agar with hemin. Growth was investigated at 30–41 °C in the presence of 0.5–1.5% (w/v) NaCl at pH 6.5–8.5. Taxonomic analysis based on 16S rRNA gene sequencing revealed that strain AM35T is affiliated with the family Muribaculaceae and closely related to the genus Muribaculum. The genomic DNA G + C content of strain AM35T was 47.8 mol%. We detected the whole-cell sugars ribose and galactose; meso-2,6-diaminopimelic acid was absent. The major fatty acids (> 10%) were anteiso-C15:0 and iso-C15:0; the major polar lipid was phosphatidylethanolamine. The major respiratory quinones were MK-10 and MK-11. Based on our phylogenetic, phenotypic and chemotaxonomic analyses, strain AM35T represents a novel genus within the family Muribaculaceae, for which we propose the name Heminiphilus faecis gen. nov., sp. nov. The type strain of Heminiphilus faecis gen. nov., sp. nov. is AM35T (= KCTC 15907 T = DSM 110151 T).

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Abbreviations

isDDH:

In silico DNA–DNA hybridization

ANI:

Average nucleotide identity.

RAST:

Rapid Annotation using Subsystem Technology

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Acknowledgements

This study was supported by grants from the National Research Foundation of Korea (NRF) (2015M3C9A4053394) and the KRIBB Research Initiative Program of the Republic of Korea.

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  1. Jun Kyu Park and Dong-Ho Chang have contributed equally to this work.

Authors and Affiliations

  1. Metabolic Regulation Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea

    Jun Kyu Park, Dong-Ho Chang & Byoung-Chan Kim

  2. 114 Bioventure Center, HealthBiome, Inc, Daejeon, South Korea

    Jun Kyu Park, Jinhoi Song & Byoung-Chan Kim

  3. Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, 99, Daehak-ro, Yuseong-gu, Daejeon, 34134, Republic of Korea

    Jun Kyu Park & Seung Bum Kim

  4. Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea

    Moon-Soo Rhee

  5. Infectious Disease Research Center, KRIBB, Daejeon, South Korea

    Haeyoung Jeong

  6. Gwanggyo R&D Center, Medytox Inc, 114 Central town-ro, Yeongtong-gu, Suwon, Gyeonggi-do, 16506, Republic of Korea

    Haeyoung Jeong

  7. Department of Internal Medicine, Chungnam National University School of Medicine, 282 Munhwa-ro, Jung-gu, Daejeon, 35015, Republic of Korea

    Bon Jeong Ku

  8. Department of Obstetrics and Gynecology, Chungnam National University College of Medicine, Daejeon, South Korea

    Mina Lee

  9. Department of Bioprocess Engineering, KRIBB School of Biotechnology, UST, Daejeon, Republic of Korea

    Byoung-Chan Kim

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Contributions

BCK and ML established the main idea of the manuscript. JKP, MSR and DHC designed the experiments and performed data analysis. JKP and BCK wrote the manuscript. The review has been checked and revised by HJ, JS, BJK and SBK.

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Correspondence to Mina Lee or Byoung-Chan Kim.

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All animal experiments were approved by the Institutional Animal Use and Care Committee of the Korea Research Institute of Bioscience and Biotechnology (KRIBB-AEC-18114).

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Park, J.K., Chang, DH., Rhee, MS. et al. Heminiphilus faecis gen. nov., sp. nov., a member of the family Muribaculaceae, isolated from mouse faeces and emended description of the genus Muribaculum. Antonie van Leeuwenhoek 114, 275–286 (2021). https://doi.org/10.1007/s10482-021-01521-x

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